Preparation Of Semiconductor Nanomaterials And Its Application In Electrochemical Luminescence Technology

Electrochemiluminescence(ECL)is a new analysis method,it not only used in chemical study but also biotechnique and sensor technology.ECL method has wide liner detection range and the detection results are accurate and sensitive.What's more,its selectivity is good.The eletrochemilumiuminescent equipment is easy to miniaturization.Semiconductor nanocomposites has unique property to fabricated ECL sensor.In this paper,there are mainly three parts based on the exploring the ECL behaviors of NH2-Gr/Au@CeO2,GO/Fe3O4/BiOI@Au and Ti-BiOBr@Au.In this paper,amidogen graphene(NH2-Gr)and Au nanoparticles(NPs)functionalized cerium oxide(CeO2)NPs(NH2-Gr/Au@CeO2)has been synthesized.The prepared NH2-Gr/Au@CeO2 exhibits strong electrochemiluminescent(ECL)activity which could be quenched efficiently by bismuth sulfide(Bi2S3).Based on this principle,a novel sandwich ECL immunosensor was constructed for the detection of prostate specific antigen(PSA).NH2-Gr was used as a loading matrix for CeO2 NPs because of its superior conductivity and large surface area.Au NPs were used to combine anti-PSA 1(AbO to CeO2 via the Au-NH2 covalent bond and meanwhile to enhance the sensitivity of immunosensor.NH2-Gr/Au@CeO2 was worked as the ECL response layer.Ag NPs functioned Bi2S3 was used to label secondary antibody(Ab2).After a sandwich-type immunoreaction,a remarkable decrease of ECL signal was observed.The signal-off sensor showed a wide liner range response from 1 pg mL-1 to 10 ng mL-1 with low detection limit(LOD)of 0.3 pg mL-1.In this work,we propose a novel electrochemiluminescence(ECL)immunosensor for detection of insulin based on graphene oxide/ferroferric oxide/gold/Bismuth oxyiodide(GO/Fe3O4/BiOI@Au).GO/Fe3O4/BiOI@Au was synthesized for the first time,BiOI microspheres were first exploited as an ECL luminescent material and the possible ECL mechanism was explored in this work.The prepared BiOI nanocomposites exhibited good luminous performance in the presence of K2S2O8.GO/Fe3O4@Au could increase the biocompatibility and conductivity of immunosensor and meanwhile to enhance the ECL of BiOI.The proposed immunosensor showed the broad linear range(0.001-50 ng mL-1)and the low detection limit(LOD,0.33 pg mL-1,S/N=3)under the optimal experimental conditions.A novel electrochemiluminescence(ECL)signal-amplified immunosensing strategy was proposed by using gold nanoparticles(Au NPs)functionalized Ti-doped BiOBr.In this work,BiOBr exhibiting larger specific surface area,exhibited ECL activity with K2S2O4 as coreactant..In addition Au NPs,exhibited more excellent electrical conductivity and could immobilize more antibodies.Therefore,a novel ECL immunosensor based on Ti-BiOBr@Au was constructed which generated higher ECL response.To investigate the performance of the immunosensor,carcinoembryonic antigen(CEA)was chosen as a model target analyte.Under optimal conditions,the immunosensor had sensitive response to CEA in a wide linear range of 1 pg mL-1 to 10 ng mL-1 with a detection limit of 0.33 pg mL-1.