Research On Protein/polysaccharide Bilayer Carrier Material With Stimuli-responsive Property

Biomacromolecules have many promising applications in biomedical field due to their excellent biocompatibility,biodegradability and no toxicity.Gelatin,agar and alginate belong to protein or polysaccharide,which are abundant in nature.In particular,these biomacromolecules have specific stimuli-responsive properties.For instance,gelatin and agar possess thermally responsive property.Recently,researchers have paid more attention to these biomacromolecules owning to their excellent properties.In this work,agar/gelatin bilayer carrier material and gelatin/alginate bilayer carrier material were prepared by simple and environment-friendly methods by making use of the stimuli-responsive properties of these biomacromolecules.Finally,the properties and the applications of the prepared protein/polysaccharide bilayer carrier material were studied.Initially,we present a simple and environment-friendly method to generate an agar/gelatin bilayer carrier material.In this method,the thermally responsive sol-gel transitions of agar and gelatin,together with their different transition temperatures,are exquisitely employed to fabricate the agar/gelatin bilayer carrier material and achieve separated loading for various materials(e.g.,drugs,fluorescent materials,and nanoparticles)in different layers.Importantly,the resulting bilayer carrier material provides protein/polysaccharide bilayer structure with a well-defined border,which is beneficial to the loaded materials in different layers to retain their original properties(e.g.,magnetism and fluorescence),and reduce their mutual interference.Moreover,the agar/gelatin bilayer carrier material still has thermally responsive sol-gel transitions property.Besides,the loaded materials in the bilayer carrier material exhibit an interesting release behavior under the control of thermal stimulii.In addition,a gelatin/alginate bilayer carrier material was prepared by making use of the thermally responsive property of gelatin and the calcium-responsive property of alginate.The prepared gelatin/alginate bilayer carrier material is spherical,and it has a well-defined border between two layers.In addition,the gelatin/alginate bilayer carrier material also provides a protein/polysaccharide bilayer structure that can load different materials in different layers and retain the original properties of these materials.Moreover,the gelatin/alginate bilayer carrier material has stimuli-responsive property,and it can achieve controlled release of drug at different temperature and different pH.In this work,we make use of the stimuli-responsive properties of thebiomacromolecules(gelatin,agar and alginate)to geneate the protein/polysaccharide delivery system by the simple and environment-friendly method.The method not only employs the advantages of biomacromolecules,but also provides two different biomacromolecule environment(a polysaccharide layer vs a protein layer).The resulting protein/polysaccharide carrier material can be employed to load materials in different layers.Moreover,the loaded materials in the protein/polysaccharide carrier material exhibit an interesting release behavior under the control of thermal stimuli.Consequently,the protein/polysaccharide carrier material is a promising candidate for biomedical applications in drug delivery,controlled release,fluorescent labeling,and bio-imaging.In addition,this work offers new methods for the construction and application of the stimuli-responsive protein/polysaccharide carrier material.