Research Of ?PDC1 Differentially Expressed Gene Knock Out For Higher Alcohols Metabolism In Saccharomyces Cerevisiae

The metabolism of higher alcohol is a complete system in Saccharomyces cerevisiae,and it is of great significance to explore the synergistic effect of different genes on higher alcohol metabolism.We carry on differential genes expression analysis after knockout PDC1,and excavate important differential genes expression quantity increase.Mutants with deletion of differential genes were constructed from the wild type strain a5 and the parental strain a5APDC1 respectively.We tested fermentation performances of the mutant strains and the parental strains,and the effect of the synergistic action of differential gene and PDC1 on the major higher alcohol products was detected.The ?5?PDC1 mutant strains n-propanol,isoamyl alcohol,actie amyl alcohol and phenethyl alcohol production decreased by 4.87%,14.46%,19.83%and 18.17%respectively,while isobutyl alcohol production increased by 69.71%.The production of n-propanol was increased by 11.05%,14.34%,8.94%and 7.25%,respectively,in the ?5?APHO3,?5?THI2,?5?THI4 and ?5?THI20.When four genetic was deleted with PDC1,the yield of n-propanol was increased by 19.45%,23.74%,19.56%and 19.49%compared with ?5.There was no significant change in the production of isoamyl alcohol in the a5 APHO3,?5 ?THI2,?5?THI4 and ?5?THI20.When four genetic was deleted with PDC1,isoamyl alcohol decreased by 23.06%,22.56%,22.34%and 22.56%,respectively.Active amyl alcohol and phenylethyl alcohol production also show similar changes with isoamyl alcohol.There was no significant effect on the n-propanol production in the mutant ?5?CHA1,and the isobutanol production was 5.74%lower than a5.When the CHA1 was deleted with PDC1,n-propanol production was increased by 16.02%,isoamyl alcohol decreased by 9.83%than parent strain ?5?PDC1.Compared with ?5,n-propanol production was increased by 21.67%,isoamyl alcohol,active amyl alcohol and phenylethyl alcohol production decreased by 22.87%,24.25%and 19.49%respectively.There was no significant effect on the major higher alcohols production in the a5AAGP1.When the AGP1 was deleted with PDC1,n-propanol production was increased by 19.25%,isoamyl alcohol and phenylethyl alcohol decreased by 9.00%and 13.45%than parent strain a5 APDC1 respectively.Compared with ?5,n-propanol production was increased by 24.05%,and the isoamyl alcohol,active amyl alcohol and phenylethyl alcohol production decreased by 22.16%,23.71%and 29.17%respectively.There was no significant effect on the major higher alcohol production in the?5?TIR2.When the TIR2 was deleted with PDC1,n-propanol production was increased by 13.72%,isoamyl alcohol production decreased by 7.29%.Compared with a5,n-propanol production was increased by 19.26%,and the isoamyl alcohol,active amyl alcohol and phenyl alcohol production was decreased by 20.69%,23%and 20.5%respectively.