| Nowadays,green development has become an important trend,and the development of green solvents in the pharmaceutical field is an important research direction.Therefore,the designal,functional,and biological deep eutectic solvent（DES）composed by quaternary ammonium salt or quaternary phosphonium salt and amine or alcohols,were applied in pharmaceuticals separation,which was the main research direction during the period of master.In this paper,we prepared a series of choline chloride based DESs and functionalized materials to investigate the bioactive molecules separation.This dissertation consists five chapters:In chapter 1,introduction.The research background of DESs and functionalized materials is summarized,and the research progress in the extraction and separation of bioactive molecules is emphasized.In chapter 2,separation and purification of palm polyphenols by choline chloride（ChCl）deep eutectic solvents（DESs）.ChCl-DESs were evaluated for the pretreatment of palm samples in the analysis of polyphenols,such as protocatechuic acid,catechins,epicatechin,and caffeic acid.During the enrichment step of the pretreatment,eight DESs were prepared and applied to the reflux extraction of polyphenols from palm samples.The ChCl DES-modified adsorbents were characterized by Fourier transform infrared spectroscopy,thermal gravimetric analysis,scanning electron microscopy.All the DESs exhibited higher polyphenol extraction efficiency than methanol,and the highest extraction efficiency was obtained using ChCl-FA（1:1,mole ratio）.For the purification step of the pretreatment,the highest purification efficiency was obtained using the Ch Cl-Ph DES-modified adsorbent as the SPE packing material and ChCl-Urea-H2O（1:1:5,mole ratio）,ChCl-Gly（1:1,mole ratio）,ChCl-FA-H2O（1:1:5,mole ratio）,and H2O as the eluents.In chapter 3,separation of radix asreris polyphenols by ternary ChCl DES based MIPs.A molecularly imprinted polymer based on a ternary deep eutectic solvent comprised of choline chloride/caffeic acid/ethylene glycol was prepared.The caffeic acid in the ternary deep eutectic solvent was used as both a monomer and template.The ternary DES-based MIPs were characterized by fourier transform infrared spectroscopy,elemental analysis,scanning electron microscopy,and atomic force microscopy.The effects of time,temperature,ionic strength,and pH on the recognition processes for four polyphenols（caffeic acid,protocatechuic acid,catechin,and epicatechin）by 13 ChCl-CA-EG ternary DES based MIPs was investigated.The results show that MIPs exhibited much higher specificities for CA because of its template role in the preparation of the MIPs.The MIP recognition processes fitted a second-order kinetic model and the Freundlich isotherm model,which indicated that the interactions between the MIPs and the polyphenols are mainly chemical interactions.In addition,the ternary DES-based MIPs were successfully applied to the recognition of CA in a real sample.This work provided a new method for overcoming the issue of insoluble template molecules during MIP preparation.In chapter 4,separation ofβ-lactoglobulin from milk by nano-biological DES-MIP@Fe3O4@MoS2.A deep eutectic solvent based magnetic molecularly imprinted polymer on the two-dimensional materials of molybdenum disulfide（DES-MIP@Fe3O4@MoS2）withβ-lactoglobulin（β-LG）template has been synthesized and the morphological characteristics,adsorption property and biological activity has been evaluated.As a result,the9-DES-MIP@Fe3O4@MoS2withsphere-likeandgoodthermalstability.9-DES-MIP@Fe3O4@MoS2 exhibited much higher specificities forβ-LG because of its template role in the preparation of the MIPs.The MIP recognition processes fitted a second-orderkineticmodelandtheLangmuirisothermmodel,the9-DES-MIP@Fe3O4@MoS2 show good stability and renewability.In addition,the9-DES-MIP@Fe3O4@MoS2 exhibited strong antibacterial activity to the bacteria growth and could restrain their reproduction.In chapter 5,conclusions and expectation were made. |
