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Salting Out Extraction Of The Main Effective Components From Sea Buckthorn Seeds

Posted on:2019-06-12Degree:MasterType:Thesis
Country:ChinaCandidate:W P SunFull Text:PDF
GTID:2371330566992027Subject:Chemical engineering
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Seabuckthorn(sea-buckthorn),also known as acid thorn,is an annual deciduous shrub or small tree of the genus Seabuckthorn.It is planted in the central and western regions of China.It has good drought resistance and sand resistance.It is widely used in soil and water conservation and desert greening.In addition,seabuckthorn is rich in vitamins,oils and phenols and other bioactive substances,which is widely used in clinical medicine,food and cosmetics.At present,the study of seabuckthorn mainly focuses on the study of the components of seabuckthorn juice extract.In this paper,the extraction of polyphenols,flavonoids and proanthocyanidins from seabuckthorn fruit residue by salting out extraction technology and the mechanism of extraction of the main effective components in seabuckthorn seed are preliminarily studied.First,the main active ingredients in Seabuckthorn pomace were extracted by two-phase aqueous extraction.The effects of the amount of organic solvent and inorganic salt,the ratio of material to liquid,the time of statics and the static temperature on the extraction of polyphenols,flavonoids and proanthocyanidins in the fruit residue were investigated.The results showed that the extraction rates of polyphenols,flavonoids and procyanidins were the maximum values of 57.56 mg/g,5.75 mg/g and 43.19mg/g,and the recovery rate was 96.63%,91.10%,respectively,88.4 min,160 eyes,1:45,35℃ and 27%(w/w)acetone/14%(w/w)citric acid.98.87%.Compared with microwave and ultrasonic extraction,aqueous two phase extraction has higher extraction rate and better antioxidant activity for polyphenols and procyanidins.Secondly,three liquid phase extraction of polyphenols,flavonoids and procyanidins from Hippophae rhamnoides seed was studied.The three liquid phase extraction process was optimized by the single factor experiment of visual number,material liquid ratio,statics time and static temperature.The system consisted of 12%(w/w)n-hexane/24%(w/w)acetone/17%(w/w)ammonium sulfate,160 eyes,the ratio of material to liquid was 1:40,the static temperature was 40℃,and the statically placed time was 60 min,and polyphenols and classes were used.The yield of flavonoids was 110.66 mg/g and 6.16mg/g respectively,the recovery rate was 94.16%and 95.32%,and the yield of proanthocyanidin was 91.56 mg/g.Thirdly,microwave assisted three liquid extraction of polyphenols,flavonoids and total sugars from seabuckthorn seeds was studied.The effects of microwave time,microwave power and static time on the distribution of effective components in three liquid phase system were investigated.The results showed that the three liquid phase salting out extraction system was 14%(w/w)n-hexane/28%(w/w)acetone/15%(w/w)ammonium sulfate,microwave power 270 w,microwave time 30 s,and stationary 60 min.Under the optimal extraction conditions,the yield of polyphenols,flavonoids and total sugar were 116.53 mg/g,7.38mg/g,99.51 mg/g,respectively.Meanwhile,microwave-assisted three liquid extracts showed high antioxidant activity.Finally,the mechanism of two aqueous phase extraction of main active components from seabuckthorn seed was preliminarily explored.The difference of salting out system between different ratios of the same system and different proportions of different systems is discussed.The effects of the types of pH,organic solvent and salt on the phase formation and the effect of the Rohrschneider-Snyder polarity constant P’on the distribution coefficient of catechin were investigated.The results show that acetone is more capable of phase than ethanol,and the phase ability of two potassium hydrogen phosphate is weak.The system pH is influenced by the type of inorganic salt.The extraction capacity of the aqueous two-phase system is closely related to the P’value of the system.
Keywords/Search Tags:Sea buckthorn seeds, Aqueous two-phase extraction, Three-liquid-phase extraction, Polyphenols, Flavonoids
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