Biotoxicity Of Two Fluorescent Nanomaterials

The application of fluorescent nano materials in the biomedical field is increasing,especially the research in biological imaging and biological markers with the biological security problem has aroused people's attention,but the effect of biological safety of fluorescent nanomaterials and its distribution in the absence of a full understanding.Therefore,two representative fluorescent nanomaterials,carbon dots and gold clusters,were selected to study their toxicity and biological distribution in mice,which provided an important basis for the application of carbon dots and gold clusters in the field of biomedicine.Methods: in this paper,mice were selected as models.Three different concentrations of nanomaterials were exposed to mice by inhalation.They were killed on 1D,7d and 28 d,respectively.Take the venous blood for blood and blood biochemistry to evaluate toxicity of nano materials as samples;take heart,liver,spleen,lung,kidney and brain tissue sections and H&E staining to evaluate the tissue damage,and electron microscopy to observe the cell injury;the liver,lung,kidney and brain frozen section and tissue homogenate measuring the light absorption intensity should be evaluation of nano materials in vivo biodistribution.Results: different concentrations of carbon dots and gold clusters after inhalation exposure to mice,at the carbon point concentration of 2mg/kg,5mg/kg,the survival rate of mice decreased,while the carbon point concentration was 1 mg/kg,no mice died.The mice in the experimental group of the gold cluster were all dead.The white blood cell indexes of the blood routine data and the experimental group were higher than those of the control group,and the other indexes were at the normal level.The level of ALT and AST in the experimental group was significantly higher than that of the control group,while the level of BUN and CREA was not significantly different from that of the control group.By histological observation,it was found that there was a different degree of injury in the liver of mice at high and middle concentrations,and the degree of injury was positively correlated with the concentration of inhaled material.The fluorescence intensity of each tissue was measured by tissue homogenate.It was found that the peak values of carbon dots in lung,liver,kidney and brain tissues were all higher than those in control group.Conclusion: through inhalation,when the carbon concentration was low,the mouse has no toxicity,but with the increase of concentration,to enhance the toxicity of mice,and the main effect in mice liver;and carbon can be discharged through the liver and kidney channel part;gold clusters have some damage to the organization but the damage is not fatal.In a word,nanofluorescence has a certain toxic effect on mice,and the toxic effect is positively related to the concentration.This study provides important experimental data for the clinical application of carbon dots and gold clusters in biology,and provides a necessary basis for establishing a biosafety and positioning platform for fluorescent nanomaterials.