Electrochemical Imprinted Detection Of Dopamine Based On A Novel Graphene And Molybdenum Selenide Composite

As an important neurotransmitter,dopamine levels in the human body can directly affect human health and lead to various diseases.With attentions on healthy life strengthened,it has been a general trend to develop a simple,efficient dopamine detection technology.In this paper,three different nanomaterials modified electrodes were prepared based on graphene and molybdenum selenide to obtain three novel electrochemical sensors that can be used for dopamine detection.It has been successfully applied to rapid and sensitive analysis of dopamine in human serum.The three sensors exhibited a broad linear range and low detection limit for dopamine assay.The appreciable stability and reproducibility are obtained and confirmed the potential to be practical application.Three parts are listed in following:?1?A novel hydrothermal route was used to synthesize of few layers S-MoSe₂/SG nanocomposites.The composite modified glassy carbon electrode was prepared as a sensor working electrode.S-MoSe₂ has a catalytic effect on dopamine,and SG,as a modified graphene material,can improve the conductivity of the material and improve the detection performance of the sensor.This sensor was applied to dopamine detection,and obtained a wide linear range(1×10^-6-1×10^-3 mol/L)and a lower detection limit(9.7×10^-8 mol/L)with good repeatability and stability.?2?The S-MoSe₂/SG composites were prepared by hydrothermal method and S-MoSe₂/SG/MIP composites were prepared by polymerizing a layer of polypyrrole molecularly imprinted layer on S-MoSe₂/SG by chemical polymerization.A glassy carbon electrode modified by S-MoSe₂/SG/MIP composites was prepared as a sensor working electrode.Differential pulse voltammetry?DPV?was used to detect dopamine,and a wide linear range(1×10^-6-1×10^-3 mol/L)was obtained with lower limit of detection(8.2×10^-8 mol/L).Due to the specific recognition of dopamine by molecular imprinting,the sensor has a better selectivity for the detection of dopamine.Its repeatability and stability tests also exhibit better repeatability and stability.?3?After preparing the S-MoSe₂/NSG nanocomposite by hydrothermal method,S-MoSe₂/NSG/Au was obtained by reducing chloroauric acid in situ on S-MoSe₂/NSG to produce gold nanoparticles.A layer of polypyrrole molecular imprinted on S-MoSe₂/NSG/Au was prepared to synthesize S-MoSe₂/NSG/Au/MIP composite.A sensor working electrode was fabricated with these composites.Differential pulse voltammetry?DPV?was introduced to detect dopamine and a wide linear range was obtained(5×10^-8-1×10^-3 mol/L).The detection limit was as low as 2.0×10^-8 mol/L.The sensor has good selectivity,repeatability and stability.Researches have demonstrated that the sensor prepared in this paper can be used as a rapid,sensitive and highly selective dopamine detection sensor Both its reliability and portability are suitable for use in practical applications.