Non-linear Dynamical Behavior And Analysis Of Thiol-Amide Self-Assembly System

Networks of organic chemical reactions are important in life and probably played a central part in its origin.Network dynamics regulate cell division,circadian rhythms,nerve impulses and chemotaxis,and guide the development of organisms.Out-of-equilibrium networks of chemical reactions have the potential to display emergent network dynamics such as spontaneous pattern formation,bistability and periodic oscillations.Therefore,the self-assembled nonlinear dynamic control network of bio-related organic substances may play an important role in the early life origin.The study of the dynamic process of its regulatory network has important implications for understanding natural phenomena and life phenomena.The thiol-amide reaction system is a typical biologically-related organic reaction network.Due to the particularity of biochemical-related substances,a microfluidic CSTR reaction and detection system has been designed.The system is divided into one-way and multi-way,which can achieve light disturbance and other controls,the system can be widely used in nonlinear dynamic related fields,can save reagents,accurate detection,and provide a broad experimental platform and design ideas for the exploration of nonlinear dynamic systems.Experiments at different flow velocities have shown that: as the velocity changes,the oscillating waveforms are varied,and the intervals,periods and amplitudes of the oscillations are greatly different from those of Semenov’s experiments.At the same flow rate experiments,found that even under the same experimental conditions,completely different oscillation waveforms can be made.It may be due to the difference of subtle conditions be amplified by the system.By changing the flow rate of the feedback agent,it was found that the single-peak interval of the oscillation can be controlled,period cannot be controlled,and the amplitude can be controlled to be shifted downward.By studying the experiments at different reaction temperatures,it is found that temperature changes have a significant effect on the oscillation,but the effect is not regular.By studying the perturbation of the 254 nm UV light to the oscillation system,it was found that the 254 nm wavelength has a perturbation effect on the oscillation,the UV light disturbance has time delay,and the result of disturbance is not uniform.So,UV light affects the stability and development of the system,the system is rich in nonlinear dynamic behavior.In-depth analyze the reaction mechanism of the thiol-amide oscillating system.Experimental study of DL-acetyl homocysteine thiol lactone,diamine maleate replace the original initiator and feedback agent to design a new oscillation system,and the effects of intermediate regulation and UV light regulation were studied experimentally.We conclude that the initiator is an organic compound characterized by thioesters.The feedback agent is a hydrocarbon or other substance characterized by a carbon-carbon double bond.Intermediates need to contain two of hydroxyl,amino and thiol groups at both ends.Addition of intermediates and UV disturbances can achieve a purposeful regulation of the oscillating system.By replacing the main reactants of the original oscillating system,found that a large number of new oscillations can be made.