| With the rapid development of science and technology,there are a variety of products that increase people’s chances of undergoing radiation and seriously threaten human health.Therefore,it is imperative to study the mechanism of radiation damage and find new and effective radiation protection agents.At present,anti-radiation studies of polysaccharides from Auricularia auricula are mostly focused on evaluating their ability to scavenge free radicals in vitro and their effects on antioxidant enzymes in the body.In this study,based on the study of Auricularia auricula polysaccharide extraction,modification and in vitro antioxidant activity in front of the research of our research group,the modified Auricularia auricula polysaccharide sulfate was subjected to radiation protection research in vivo,and the radiation resistance mechanism was studied from the biochemical and genetic levels.Research provides theoretical support for the value of polysaccharides in Auricularia auricula.In this study,the Auricularia auricular polysaccharide sulfate(SNAAP)obtained by modifying Auricularia auricula with chlorosulphonic acid-N,N dimethylformamide was used as a test substance to establish a 60Co-γ radiation injury model.The results showed that different doses of SNAAP can relieve the damage of 60Co-γradiation to the body’s immune system,hematopoietic function,oxidation balance and bone marrow DNA in a certain degree,and play a role in radiation protection.Among them,the high-dose antiradiation effect is the best.The specific protection pathways are as follows: SNAAP can promote healthy growth of mice,increase body weight,increase related immune organ index,promote splenic lymphocyte proliferation,increase IL-1β,IL-6,IL-10,TNF-α,IFN-γ,Ig M The content of immune factors,such as LTB4,TGF-β content,thus enhancing the immune ability of mice,and improving radiation resistance;SNAAP by increasing the number of peripheral blood lymphocytes,white blood cells,red blood cells and platelets,increasing the DNA content in the bone marrow Anti-radiation effect;SNAAP can significantly reduce lipid peroxide MDA content,increase SOD,GSH-px,CAT no vitality,by maintaining the body’s antioxidant-antioxidant system balance play a role in radiation protection.In this study,normal,model and SNAAP-H mouse spleen were used as experimental materials to construct mi RNA library of spleen tissue.dentifying known mi RNAs through deep sequencing and predicting new mi RNAs;using bioinformatics to compare and analyze differentially expressed mi RNAs between the model group and the blank group,the high-dose administration group and the model group,Target genes were predicted and target genes were subjected to GO enrichment analysis and KEGG analysis.mi RNAs,target genes and pathways related to organism immunity were screened;q RT-PCR was used to verify selected mi RNAs and target genes.More than 600 known mi RNAs were identified,and more than 180 new mi RNAs were predicted.Among them,known mi RNAs predicted about 2.1 × 104 target genes,and the new mi RNAs predicted about 1.8 × 104 target genes.radiation was found to cause the upregulation of mmu-mi R-200a-3p,mmu-mi R-200b-3p,mmu-mi R-200c-3p,and mmu-mi R-375-3p,and after administration of SNAAP for protection,The expression of mmu-mi R-200a-3p,mmumi R-200b-3p,mmu-mi R-200c-3p,and mmu-mi R-375-3p was down-regulated.For new mi RNAs,radiation causes upregulation of mmu_novel_mir307 and downregulation of mmu_novel_mir322.After SNAAP administration,the expression levels of mmu_novel_mir307 and mmu_novel_mir322 decreased to some extent.Through bioinformatics analysis,t Screening the known differences of mmu-mi R-200a-3p and mmu-mi R-200c-3p,and predictions by mi Randa software showed that mmumi R-200a-3p interacts with Cytokine-cytokine receptor interaction pathways.Gene Ifnar1 has positive regulation;mmu-mi R-200c-3p positively regulates CCR5 and il1r1 genes in the Cytokine-cytokine receptor interaction pathway and positively regulates smad5 gene in the TGF-beta signaling pathway. |
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