The Analysis Of Muscle Fiber Types And Culture Of Satellite Cells In Vitro Of Alxa Bactrian Camel

The Alxa bactrian camel can be divided into the gobi camel and desert camel.The gobi camel always live in vast gobi and the desert camel live in the hinterland of desert.The gobi camel has shown an obvious advantages over the desert camel in terms of meat production that the reason is unclear.In this paper,Different ages(6,8,10)gobi camels and desert camels were used to compare the slaughter performance and to stain the MyHC muscle fiber in different muscle(The triceps muscle of arm,the longissimus muscle,the biceps muscle)by ATP enzyme staining.The percentage of different MyHC was determined,and histological characteristics were analyzed.In addition.The author ultilizes the method of two-step enzyme digestion and the way of tissue block to cultivate desert camel satellite cells.Moreover,The author use a differential adhesion method to make a purification for the satellite cells.And also the experiment chooses different concentration gradients to make sure the optimum digestion time of trypsin,In the end,the author make Immunofluorescence staining identification by using the satellite cell marker genes Pax7(paired box transcription factor)and Myo D1(myogenic differentiation factor),The results showed:The live weight of Gobi camel is about 60 kg heavier than that of desert camel,and its carcass weight is about 80 kg higher than that of the desert camel.Its net meat weight is about 90 kg higher than that of the desert camel,there was significant difference among different age groups(P<0.05;P<0.01).At 6 age,the contents of MyHC I and MyHC IIb of Gobi camel in triceps muscle of arm were less than that in desert camels,and opposite to MyHC II a(P<0.05);At 8 age,he content of MyHC I in Gobi camel is less than that in desert camel,but MyHC II A and MyHC IIb are higher than those of desert camel(P<0.05);At age 10,the contents of MyHC I and MyHC IIb in Gobi camels were high,and MyHC IIa content was lower than that in desert camels(P<0.01).At 6 age,the content of the MyHC in longissimus muscle was the same as that of the triceps muscle of arm.At 8 age,the content of MyHC was opposite to that in triceps muscle of arm.At age 10,MyHC I in Gobi camel was higher than that in desert camel,but the content of MyHC II A and MyHC II B were lower than those in desert camel(P< 0.01),and the change rule was the same at 8 age;at 6 age,In the biceps muscle,the contents of MyHC I and MyHC II A in Gobi camel were higher than those in desert camel,and it was opposite MyHC II B(P<0.05).At age 8,the contents of MyHC I and MyHC II B in Gobi camel were lower than those in desert camel and MyHC II a(P < 0.05).At age 10,the content of MyHC I in Gobi camel was higher than that in desert camel,but MyHC II A and MyHC II B were opposite(P<0.01).In both triceps muscle of arm and longissimus muscle,the fiber diameters of the three type fiber in Gobi camel are generally larger than that of the desert camel(P <0.05),;the diameters of MyHC in the longissimus muscle are generally larger than that in triceps muscle of arm and Biceps muscle;the density of MyHC Gobi camel is generally higher than that of desert camel(P <0.05),and the density of MyHC II type is the largest.The diameter of MyHC in Gobi camel has the greatest differences at 6 age,the smallest difference is 10 years old(P<0.05);MyHC density were generally greater than that at 8 years old and 10 years old.That the satellite cells obtained by the tissue culture method and the differential adhesion method have a good morphology,a strong refraction and a high purity,and it is the most effective method for culture of satellite cells in vitro;It was concluded that the best digestion time of the cells was 8min-12 min through trypsin concentration gradient experiments;The Myo D1 and Pax7 satellite cell marker immunofluorescence staining experiments indicated that Myo D1 and Pax7 both were positive expression.