Preparation And Antitumor Cell Activity Evaluation Of The Surface Pro-drug Modified Nano-size Erythrocytic Vesicle

One of the themes of nano drug delivery vehicle design is safety,and the second is high efficiency.In recent years,biomaterials having good biocompatibility and biodegradability have been used as basic materials for nano-drug delivery system,which can not only enhance the drug therapeutic effacy,but also significantly reduce the side effects.However,simple degradable carriers often fail to meet the needs of the complex physiological environment in humans.A large number of research results and clinical manifestations reflect that the nanoparticle loaded with anticancer drugs using the erythrocyte capsule has a good tumor-delivery function and biocompatibility through a liposome squeezing device,and has the research and application for clinical tumor targeted therapy.Great potential and value.Therefore,this article designs and synthesizes the polymer prodrug DSPE-PEG-Ir,and binds to the surface of the erythrocyte capsule through the inserted form,so as to improve the recognition and destruction of the nano drug by the body’s immune system in order to increase the biological safety of the vector to a greater extent.Sex and drug treatment efficiency.Therefore,the research combined with "autologous" and "artificial" materials for the construction of multifunctional nano-drugs can achieve efficient and safe anti-tumor therapy.This study consists of the following two components: First,anti-tumor prodrugs: DSPE-PEG-Ir;Second,nano-administration of carriers: drug-loaded nano-erythrocyte microcapsules(DSPE-PEG-Ir@RBCNV).The main research contents and conclusions are as follows: The one-step esterification reaction was used to synthesize the anti-tumor polymer prodrug DSPE-PEG-Ir,and its structural characteristics were confirmed by nuclear magnetic resonance spectroscopy to verify the successful preparation of the prodrug.Preparation and characterization of prodrugs inserted into erythrocyte microcapsules and DSPE-PEG-Ir@RBCNV.The polymer prodrug DSPE-PEG-Ir was co-incubated with the erythrocyte envelope to allow insertion of DSPE-PEG-Ir into the erythrocyte capsule.The drug-loaded nano-erythrocyte microcapsules(DSPE-PEG-Ir@RBCNV)were prepared by repeated extrusion using a liposome extruder.Zeta-DLS and TEM test results show that DSPE-PEG-Ir@RBCNV has a uniform spherical nanostructure and good dispersion characteristics.In vitro drug release results show that it has controlled release of pH-sensitive drugs.The effect of DSPE-PEG-Ir@RBCNV on synergistic antitumor activity was evaluated using in vitro cell assays.The experimental results show that DSPE-PEG-Ir@RBCNV exhibits excellent anti-macrophage phagocytosis,which provides an important basis for promoting long-acting blood circulation and achieving targeted,safe,and efficient tumor drug delivery.In addition,DSPE-PEG-Ir@RBCNV can enhance synergistic anti-tumor efficacy by promoting tumor cell endocytosis.