Controllable Preparation Of Ordered Nanostructure And Its Biological Applications

Ordered nanostructures exhibit unique properties and have a strong potential for the application in the field of biology.The coinage metal nano-array structure is a kind of highly efficient surface-enhanced spectroscopy substrate,which exhibits surface plasmon resonance effect under the excitation of an incident certain wavelength laser.The nano-array pillar structure can be applied to the biology,which is a new type of physical antibacterial material at certain size.In this thesis,different ordered nano-array structures had been prepared basis the template methodology,and applied them to the related field of biology,such as enhanced spectroscopy detection and antibacterial material.Through the adjustment of the preparation parameters,we can realize the regulation and control of ordered nanostructures,establish the relationship between performance and structure,and finally achieve the purpose of application,the main research contents are as follows:（1）Anodized TiO₂ nanotube ends were used as template to fabricate silver nano-array structures by physical vapor deposition.The enhancement factor of the as-prepared optimized sample was over 7.68×10⁴,and the detection limit of Rhodamine 6G（R6G）was as low as 10^-1010 mol/L.The Finite Difference Time Domain（FDTD）analysis was engaged to simulate the electromagnetic field distribution on the structure surface,the calculation result and the experimental result was match well,which proved the reliability of enhancement effect.This enhancement substrate has application potential in the field of biological molecules and matters detection（2）The ordered silver nanostructure was controlled fabricated on the TiO₂ cave template by the direct current magnetron sputtering process.The Raman scattering signal enhancement factor reached 2.14×10⁶ and detection limit of R6G was 10^-1414 mol/L on the100nm-diameter silver unit sample.The fluorescence intensity 10^-55 mol/L R6G was increase to 18 times.For Mc3tc-E1 cell line fluorescence labeling,the enhancement factor was 14.Furthermore,fluorescence stability has been greatly improved,which still maintain the initial strength of 20%after 20 minutes.Such ordered nanostructure had excellent enhancement property,and could renovate the traditional biological fluorescence technique.（3）A developed anodic oxidation process was proposed to fabricate the ultra-thin and through-hole alumina nanotube and alumina nanowire arrays with controllable structure.The new route needs one step anodization only,which simplifies the preparation process.Controlling the thickness of ultra-thin and through-hole anodic aluminum oxide through sputtering time and effectively regulates the structure unit through adjusting the oxidation voltage,oxidation time,electrolyte concentration,temperature and other parameters.The as-prepared alumina nanowire array structure was applied to the physical antibacterial verification.The antibacterial effect of alumina nanowires with different morphologies was investigated.When the diameter of nanowires was 86 nm and the period was 180 nm,the antibacterial efficiency is 93.71%.