Research On Laser Processing Of MiRNA126 Biomimetic Coating Coronary Stents

Percutaneous coronary intervention(PCI)is currently one of the most effective methods for treatment of coronary heart disease(CHD).It is widely accepted due to its micro trauma and high efficiency.However,restenosis will occur in 3-5 years after surgery.The emergence of drug eluting stent has greatly delayed the restenosis cycle.Therefore,it is becoming a hot spot and direction for the study of the effect of anticoagulant drug on the surface of the stent.Through the surface modification of the stent surface by laser interference,the micro/nano structure processed on the surface of the stent will not only affect the cell activity of blood cells,but also improve the drug-loading performance of the stent.A four-beam laser interferential processing system was constructed experimentally.By adjusting the incident angle of the laser,uniform and ordered pit micro/nano structures with periods of 5 ?m,10 ?m,and 15 ?m were prepared on the surface of coronary stents.Then,phosphorylcholine and miRNA126 were respectively loaded onto the surface of the stents with three kinds of micro/nano structures and no process by means of dielectrophoresis and electrostatic spraying.The distribution of the drug coating could be observed by scanning electron microscopy(SEM).And by comparing the drug loading of the four kinds of drug coatings and the robustness in the simulated blood system,the experiment finally found that the micro/nano structures on the surface of the stents can increase the drug loading of the stents,And in three cycles,the stent with the 10 ?m cycle is the best in the overall performance.This provides a direction for the research area of the restenosis after interventional therapy.