Development Of Biofunctionalized AgInS₂ Quantum Dots Based On Microfluidic Technology

The incidence of breast cancer is the first of the female malignant tumors in China,but the existing methods are currently limited in age,examination times and sensory accuracy.Therefore,the detection methods of breast cancer need to be improved.Ternary AgInS₂ quantum dots are are potential fluorescent probes for breast cancer cells.Because of their advantages of low toxicity and many synthetic methods,it has been widely used not only in biological detection but also in photovoltaic industry in recent years.However,the traditional hydrothermal method for the preparation of AgInS₂quantum dots has the disadvantages of long-time consuming,more required drugs and weak fluorescence intensity,and it needs complex modification process before application in biological field.The emerging microfluidics technology can set all functions like transferring samples,controlling the volume of chemical products,mixing the reagent and heating in one chip,and improve the above defects in synthesizing quantum dots.In this paper,microfluidic chip technology is applied to synthesize biologically functionalized AgInS₂ quantum dots directly,and AgInS₂quantum dots are applied to breast cancer cell imaging research.In this paper,based on the experimental characteristics of AgInS₂ quantum dots prepared by hydrothermal method,a Y microfluidic chip containing snake channel is designed,and the simulation results of COMSOL Multiphysics software are combined to obtain the reaction temperature of the AgInS₂ quantum dots and the width of the chip channel to make the microfluidic chip,through which the ternary FA-AgInS₂ quantum dots with good biocompatibility were prepared.The optical properties,cytoactive and fluorescence imaging of nanocomposites were studied by means of UV-VIS-NIR spectrophotometer,MTT colorimetric assay and fluorescence inverted microscope.The results show that,under the same Ag⁺/In³⁺molar ratio,the FA-AgInS₂ quantum dots synthesized by microfluidic have the advantages of high fluorescence intensity,low cytotoxicity and strong biological compatibility.Therefore,the FA-AgInS₂ quantum dots synthesized by microfluidic chip have a good application prospect in the field of bioimaging research,which has laid a good experimental foundation for the rapid detection of breast cancer in vitro.