Design,Synthesis And Application Of Cyclometalated Iridium Complex

With the advancement and development of social science and technology,people’s awareness of green and healthy life is constantly increasing.Life and health are affected by many factors in the natural environment,such as Fe²⁺,Cu²⁺,I^-,glucose,amino acids,H₂S and other substances which are closely related to life and health.Therefore,it is of great significance to be able to detect these ions in a substances quickly and accurately way.Phosphorescence probes have been widely applied in biomedicine due to their advantages of simple operation,accurate detection,and low price.In this paper,two kinds of the cyclometalated iridium（?）complexes with different structures were designed and synthesized.One is based on the electrostatic adsorption of ClO₄^-and the cationic iridium（?）complex to realize the function of detection of ClO₄^-which is named IrIm;Phosphorescent probes IrOC for detection of H₂S is based on the addition elimination reaction of olefinic hydroxy functional groups with H₂S.1.The phosphorescent probe IrIm detecting to ClO₄^-.We design,synthesis of the aggregation induced luminescence phosphorescent probe based on the electrostatic adsorption action.The probe uses two imidazole groups to increase the water solubility and positivity.Experiments show that the probe IrIm is a near-infrared water-soluble probe.Only a weak red light in the aqueous solution which the luminous intensity increases 250 times when added ClO₄^-.The probe IrIm has a specific phosphorescent response to ClO₄^-in the presence of other anions.SEM test,TEM test and DLS test also showed that the probe IrIm formed about 40 nm nanoparticles when added 7 times ClO₄^-leading to aggregation-induced luminescence.Cell experiments also show that the probe can detect ClO₄^-well in cells.2.The phosphorescent probe IrOC detecting to H₂S.The specific addition eliminate reaction is happened with the probe IrOC because of the nucleophilicity of H₂S.The process is certified by HRMS and ¹H NMR.It is found that the peak of m/z 797.1736（mass-to-charge ratio of IrOC is 797.1740）disappeared after the addition of H₂S,and two new peaks of m/z689.1549 and m/z 743.1656 are generated（mass-to-charge ratio of IrOH is 689.1529,IrOC-OH mass-to-charge ratio of 743.1634）.Titration experiment shows that the maximum response of the probe IrOC to H₂S can be enhanced by 120 times.It is proved that the probe IrOC has a good phosphorescence response to H₂S under the interferenced of other anions and bio-mercaptans.Cell experiments showe that probe IrOC can successfully enter into HeLa cells and response to H₂S.The results of the H₂S test in the gas shows that the probe IrOC can also respond to H₂S in the air.