Preparation Of Aqueous Transition Metal Dichalcogenides And Application In Immunoassay

Low dimensional transition metal dichalcogenides?TMDCs?possess some excellent features,such as large specific surface area,strong adsorption capacity,and good biocompatibility,which make them exhibit good application prospect in biomedicine and other fields.This thesis mainly focuses on the optimization of the Raman characteristic of TMDCs micro-nano materials and high performances Raman based immunoassays.The results are as follows:?1?The water phase WS2 and MoS2 micro-nano materials with strong Raman scattering signal excited by a visible laser are obtained.It is found that the matching degree of Hansen solubility parameter between the solvent and TMDCs has a great influence on the Raman intensity of TMDCs obtained by the liquid-phase exfoliation method.Simultaneously,the appropriate heat treatment?the optimum is 70??can optimize the crystallization quality of the samples,which further enhanced the Raman scattering intensity.?2?High specific immunoassays of human IgG were realized using the MoS₂ based Raman probes,with a low experimental detection limit of 1fM.On this basis,dual channel detection with high sensitivity were realized,where MoS2 and WS2 were used to label antibodies specifically identified with two different analytes.The detection limit of double channel detection is 1nM.?3?The Raman scattering of MoS₂ was enhanced by the modification of the noble metal nanoparticles and an immunoassay with a lower detection limit?0.6fM?was finally obtained.The current sensitive Raman based immunoassay under visible light excitation can greatly reduce the cost,improve the safety of operation,and promote the application of Raman detection technology.