Synthesis Of Fatty Acid Methyl Ester From Pistacia Chinensis Seed Oil Catalyzed By Magnetic Cellulose Microspheres Immobilized HPW

In this study,Pistacia chinensis seed oil was treat as material for synthesis of Fatty Acid Methyl Ester(FAME).The magnetic cellulose microsphere(MCM)was prepared and used as substrate to immobilize H3PW12O40(HPW).Then an efficient clean microwave-assisted method of synthesis of FAME from Pistacia Chinensis seed oil catalyzes by MCM-HPW was formed.The experimental works and conclusions of this study are as follows:1.An Efficient microwave-assisted extraction method was proposed and applied on the extraction of Pistacia Chinensis seed oil.Microwave radiation can increase the power of material,intensify the motion of molecule and lead to the rapid cell rupture.Then oil outflows easily.In this study,microwave radiation was used to extract Pistacia Chinensis seed oil.In the experiments,the extraction conditions was first studied by single factor experiment and then optimized by orthogonal test.The optimal parameters of microwave-assisted extraction of Pistacia Chinensis seed oil was as follow: extraction solvent petroleum ether,solvent/material ratio 6:1(mL/g),microwave power600 W,extraction temperature 50 ℃,extraction time 40 min.Under the optimal extractionconditions,the extraction yield of Pistacia Chinensis seed oil seed oil was 37.0%.Then,the main physicochemical properties of Pistacia Chinensis seed oil were determined using GB/T standard methods.It indicated that there was little difference of the main physicochemical properties between Pistacia Chinensis seed oil and common FAME feedstocks.So the Pistacia Chinensis seed oil was suitable as an alternative raw material of FAME.2.The preparation and characterization of magnetic cellulose microsphere immobilized HPW catalyst.In this study,the thermosol technology has been used to prepare regenerative cellulose microsphere(CM),and the CM was grafted by epichlorohydrin and TETA.Then,magnetic Fe3O4 was synthesized in the micropore of CM via co-precipitation reaction.Afterwards,the HPW was immobilized on MCM by chemical bonding with amine groups to form MCM-HPW catalyst.The immobilized catalyst was characterized by FTIR spectroscopy,scanning electron microscopy,X-ray diffraction.Results showed that the cellulose substrate was uniform spherewith abundant pore structure.And all characterization results showed that HPW was successfully immobilized on the magnetic cellulose substrate.The loading content of HPW on MCM-HPW catalyst was determined by ICP-MS,and 19.6% of loading content was obtained.Meanwhile,the immobilization of HPW was uniform and stable.3.The magnetic cellulose microspheres immobilized HPW catalyzed transesterification reaction to synthesize fatty acid methyl ester from Pistacia Chinensis seed oil and the kinetics study.The magnetic cellulose microspheres immobilized HPW catalyst was applied on the production of FAME.And microwave-assisted catalyzing was employed in the process.The effects of conditions on conversion yield were investigated through single experiments.Then,the optimal conditions of microwave-assisted FAME production from Pistacia Chinensis seed oil catalyzed by HPW have been gained: Catalyst amount 15%,Methanol/oil volume ratio 10:1,Reaction temperature 60 ℃,Microwave power 600 W,Reaction time 80 min.Under the optimal conditions,the conversion yield reached 93.1%.The prepared catalyst can be speared easily by a magnet and recycle at least 4 times.The reaction mechanism and kinetics were also researched.It was found that the reaction was first-order kinetic reaction.The reaction rate constant of different temperatures and theactivation energy were calculated respectively.Meanwhile,the main FAME compositions of Pistacia chinensis seed oil were analyzed by GC-MS,their relative content respectively were methyl palmitate 18.04%,methyl linoleate 33.48%,methyl oleate 46.73% and methyl stearate1.46%.