Synthesis Of Different Morphology Copper Sulfide By Interface Reaction And Mitochondrial Targeted Drug Delivery

Inorganic micro-nano materials can be commonly prepared through a variety of methods,including solvothermal method,sol-gel method,hard template method,soft template method,pulsed laser deposition method,and interfacial reaction method.Inorganic micro-nano materials are widely used in various fields such as fluorescent labeling,drug delivery,sewage treatment,chemical energy storage,and catalysis.With the rapid development of nanoscience and nanotechnology,the application potentials of inorganic micro-nano materials in drug delivery and tumor treatment attracted tremendous attentions in recent years.Liquid-liquid two-phase interfacial reactions for the controllable preparation of inorganic micro-nano materials provide a new way of thinking.This preparation method can be easily preformed under mild conditions.Futhermore,the morphology and size of the products can be efficiently controlled.It can be used to prepare various types of materials,such as metal elements,metal oxides,sulfides,carbonates and other materials.This preparation method mainly consists of several steps,?1?dissolving the organometallic precursor in a water-insoluble organic phase,?2?reacting with the reducing agent,alkali,or vulcanizing agent dissloved in the aqueous phase at the interface between the two phases under a certain temperature,and?3?self-assembling at the interface.This method can be applied to easily prepare some inorganic micro-nano materials with good dispersibility,special morphology,hierarchical assembly or low-dimensional structures.This thesis is divided into the following aspects:Using sodium diethyldithiocarbamate trihydrate?DDTC?as organic additive,CuS single crystal with cross structure was synthesized through hydrothermal reaction and the physic-chemical properties of the product were characterized.The results show that the as-prepared copper sulfide has uniform particle size and good dispersion.In addition,the sample can significantly degrade methylene blue?MB?under visible light condition,suggesting its good application potentials in the catalytic degradation of organic pollutants.Using CuCl₂ as metal precursor and Thioacetamide?TAA?as vulcanizing agent,flaky CuS composed of small particles were successfully prepared at the interface between ether and water.The physico-chemical properties of the samples were characterized by a variety of methods.The results show that the sample has the uniform size and good stability and dispersibility in PBS buffer solution.Cell viability experiments show that the sample can lead to cell necrosis and apoptosis and it also has a certain inhibition effect on the proliferation of tumor cells.Using the water soluble fraction of pollen as organic matrix,hollow calcium carbonate nanospheres with hierarchical structure were successfully synthesized through a facile green one-pot strategy.The nhollow anospheres has a large specific surface area and mesoporous structure.These special features give it the good drug loading efficiency as 8.58%.The release of the cargo exhibits the obvious pH-responsive and sustained manner.In vitro assays show that the drug loading system can be specifically internalized by cancer cells,and targeted localized to mitochondria,which significantly augmented the apoptosis-inducing effect and the specificity of the DOX.The specificity of the DOX is significantly enhanced by17-fold through loaded onto the as-prepared hollow nanospheres.