| The physical properties of anticancer nanodrugs have a significant impact on cancer therapy efficiency.In recent years,the studies of size effect of nanodrugs on cancer therapy efficiency are based on carriers.The involvement of carriers has resulted in low drug loading and biological metabolic problems,it also changes the other physical properties of the nanodrugs,thus can not directly reflect size effect of nanodrugs.As such,we focused on the study of size effect of carrier-free nanodrugs on cancer therapy efficiency.In addition,the application of dual-mode imaging in diagnosis was also studied.The specific research contents are as follows:(1)A kind of hydrophobic anticancer drug NPs—tamoxifen(TMF NPs)with four different sizes were prepared by anodic aluminum oxide(AAO)template-assisted method,the particle sizes are 20,50,100,and 200 nm,respectively.The results show that the TMF NPs with the size of about 50 nm have the highest in vitro cytotoxicity,the highest cell uptake efficiency,and the cellular uptake mechanisms of different sizes of TMF NPs are also different;In vivo study indicated that TMF NPs with size of about 50 nm have the best tumor site enrichment effect,and ultimately has the most significant cancer treatment efficiency,the tumor inhibition rate reached 77.25%.(2)We designed a kind of nanoprobe—NPAPF-Fe3O4NPs with magnetic targeting fluorescence/magnetic resonance dual-mode imaging by using the combination of NPAPF and Fe3O4 NPs.The results show that the fluorescence intensity of NPAPF-Fe3O4 NPs is lower than that of NPAPF NPs at the same concentration in cell imaging experiments.However,due to the appropriate Fe3O4 NPs doping ratio,it can still achieve better fluorescence imaging in vitro and in vivo.In addition,in vitro magnetic resonance imaging showed a linear relationship between the 1/T2 of NPAPF-Fe3O4 NPs and the concentration of Fe,which is a T2 contrast agent capable of magnetic resonance imaging. |
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