Construction Of New Kinds Of Carriers Based On The Parinaric Acid And Studies On Their Oxidative Stability And Bacteriostasis

Parinaric acid(PnA,Conjugated octadecatetraenoic acids)has excellent physiological activity,including anticancer,hypoglycemic,inhibition of atherosclerosis,and lowering blood pressure etc.In addition,the bioactivities of Pn A was higher than that of conjugated linoleic acid(CLA),which is the most widely studied active fatty acids in food science and nutritional biochemistry at present.However,PnA shows very poor stability in the air,since it has four conjugted double bonds in the long fatty acid chain.Such poor oxidative stability then leads to greatly restricts in its related research and application.Ina ddition,it is difficult for PnA to be directly applied in the preparation of liquid food or beverage products since its water solubility is very poor.At the same time,the hydrophobic characteristics of PnA also lead to the low bioavailability.The key to solving the above problems is to construct a nutrient delivery system,which is applied to the load and delivery of bioactive substances.By constructing an efficient carrier delivery system,the water solubility,stability and bioavailability of functional molecules can be greatly improved.In view of this,this paper modifies PnA by polyol,polyethylene glycol,carboxated chitosan,chitosan and chitosan oligosaccharide as a hydrophilic group,and has synthesized a series of two affinity PnA derivatives.The purpose of the synthesis of these derivatives is to construct a new carrier material based on PnA.We first investigate the oxidative stability,the new PnA derivative fluorescence and antibacterial activity.First,the oxidative stability of each derivative was greatly improved,and there was no obvious change in the fluorescence characteristics and biological bacteriostasis compared with the free PnA.After that,we also examined the feasibility of these derivatives as a drug carrier.We are on the self assembling properties of derivatives,including critical micelle concentration,dispersion of particle size and morphology were preliminarily characterized with lutein as model molecules,investigated the new carrier based on PnA load and release of lutein.The results showed that PnA-polyethylene glycol,Pn A-amidated chitosan oligosaccharide could form good drug loaded micelles with good properties.In simulated gastric fluid,the drug loaded micelles showed good sustained release property.At the same time,the stability of the drug loaded micelles has also been greatly improved.In a word,the current research is of great significance for improving the stability and bioavailability of PnA.