Study On The Diagnosis And Drug Release Behavior Of Multifunctional Composite Nanoparticles With Atherosclerosis

Globally,cardiovascular diseases have become the primary threat to human health;Atherosclerosis is particularly severe.At present based on the traditional drug transport system can't effective diagnosis and treatment of atherosclerosis,with the progress of nanotechnology and intervention,provides the treatment of atherosclerosis with functions of targeted drug delivery methods and diagnostic tools.This undoubtedly provides a new method for the diagnosis and treatment of atherosclerosis.For the diagnosis and treatment system of atherosclerosis,the key is to select the appropriate nano-carriers and target them to specific regions to complete the drug delivery and diagnosis of the lesion area.Therefore,it is very important to use targeted nanoparticles to diagnose and treat atherosclerosis.In this paper,a kind of biophilic polymer-polyethylene glycol graft copolymer?PIA-g-PEG-g-DDA?was synthesized by free radical polymerization.The polymer contains a large number of available carboxyl groups.Through esterification reaction,we will be a kind of can be targeted to myeloperoxidase?atherosclerotic plaque of enrichment of an enzyme?target molecules of serotonin?5-HT?grafted to the PIA-g-PEG-g-DDA,preparation which has the function of target type amphiphilic polymer 5-HT-g-PIA-g-PEG-g-DDA and use it as a carrier preparation which has the function of double modal imaging contrast agents.This article selects the two contrast agents to the preparation of double modal imaging agent,respectively superparamagnetic iron oxide nanoparticles?SPION?and water soluble carbon fluorescence quantum dots?CDs?which is suitable for oil amine coated SPION nanoparticles with T₂ MRI imaging,has been used in commercial use;CDs is a carbon quantum dot with electronegativity.By solution method,SPION was coated in 5-HT-g-PIA-g-PEG-g-DDA to form the targeted nanoparticles with the function of T₂-angiography.The results of the test showed that the results showed that the magnetic resonance imaging capability of the stable nanoparticles in vitro was confirmed by MRI.The results of cytotoxicity test show that the nanoparticles have good biocompatibility.The above shows that this is a potential imaging nanoparticle for diagnosing atherosclerosis.A water-soluble fluorescence quantum dot was synthesized by microwave pyrolysis,citric acid and urea.Through characterization,the fluorescence intensity of fluorescence quantum dots is high,the nature is stable,and because it is electropositive,we can make it through the electrostatic adsorption wrapped in targeted sexual nanoparticles 5-HT-g-PIA-g-PEG-g-DDA@SPION.CDs@5-HT-PIA-g-PEG-g-DDA@SPION the double modal imaging function of nanoparticles.After debugging,the nanoparticles also retain the imaging function of two imaging modalities,and have the characteristics of low toxicity and degradation.By the method of atom transfer radical polymerization?ATRP?was synthesized with mechanical force sensitive functions nano-micelle polyacrylic acid grafted acrylic hydroxyl ethyl ester block poly(n-butyl acrylate?PAA-g-HEA?-b-PnBA,cross-linked with acrylamide formation of crosslinked hydrogel nanoparticles.In this gel,drug loading was carried out,and the therapeutic effect of the revascularized atherosclerotic plaques was tested by a self-made vascular bionic environment.The results showed that the gel showed good performance in the treatment of atherosclerosis.Designed in this paper synthesized a kind of atherosclerotic plaque diagnosis of nanoparticles,and designed a kind of environment for atherosclerosis drug release system,contribute to the diagnosis and treatment of atherosclerosis the new strength.