Preparation And Properties Of Self-assembled Liquid Crystal Folic Acid Nanoparticles

The nanoparticle targeting drug delivery system has become an effective carrier for insoluble or two amphiphilic drugs as well as targeting tumor tissue.Nanoparticles can not only reduce drug resistance and side effects through the mechanism of drug internalization,but also reduce the outflow of drugs through intracellular glycoprotein mediated.Therefore,the design of nanoscale targeting drug delivery system is an important way for scientists to study the treatment of tumor.The folic acid targeted nano-drug delivery system is a new system has significant effect for many kinds of malignant tumors.It is found that folate receptor(FR)is expressed conservatively on most normal cells,but overexpressed in many malignant tumors.Therefore,FR has become an important marker for the detection of cancer cells,and it has been widely used in the detection and treatment of targeted cancer cells in nanoparticles.Using the interaction between specific receptors and target ligand groups that are overexpressed on the surface of tumor cells,ligand carriers can fully combine with receptors to achieve specific targeting properties.The use of folic acid as a carrier to treat drugs can reduce the side effects of anticancer drugs in the body and enhance the toxicity of cancer cells.And the uptake of folic acid by tumor cells is stronger than that of normal cells.Therefore,the treatment of malignant tumor with folic acid as a drug carrier is of practical significance.The aim of this study was to prepare cell targeted self-assembled liquid crystal folic acid loaded levamisole nanoparticles,and characterize the particle size,morphology and entrapment efficiency.At the same time,cell experiments had been carried out to verify its targeting.The addition of sodium hydroxide to the folic acid solution to make folic acid self assembled to form liquid-crystal folic acid.Characterization by biomicroscope,polarizing microscope,scanning electron microscope and small angle X ray diffraction.Then the levamisole was encapsulated.When the hydroxypropyl methylcellulose(HPMC)was added to the liquid crystal folic acid solution,the two phase systems will be formed,and the nano scale dispersion domain appears,then the liquid crystal folic acid nanoparticles will be formed with the crosslinked salt.Determination of particle size and morphology by dynamic light scattering and transmission electron microscopy,Then Encapsulation of levamisole was then carried out.Finally,the drug release behavior and targeting in vitro were explored.There was a self-assembled characteristic peak at 3.3° degrees in the XRD diagram,which indicated that folic acid had been turned into liquid-crystalline folic acid.It showed a dendritic texture under the biological microscope,dendrites stacks under scanning electron microscope,it showed a layered structure under a polarizing microscope.When the mass ratio of folic acid to HPMC was 1:10,the average particle size was about 230 nm.The shape of the particles was dendritic,encapsulation efficiency was as high as 87%.Besides,Nanoparticles displayed good sustained-release performance in simulated body fluid.In cell uptake and cytotoxicity experiments,it was proved that the liquid crystal folic acid nanoparticles have the targeting properties.The self-assembled liquid-crystalline folate acid carrier nanoparticles had a high drug encapsulating efficiency,more uniform size and size distribution,excellent sustainable-relaese behavior.Also had sustained release of drugs and targetability.Therefore,self-assembled folate acid carrier nanoparticles can be used as a new targeted drug delivery systerm.