Study On Surface Of Titanium Alloy By RGD Bio-functionalization

Ti-6Al-4V alloy,as a excellent biomedical metallic material,has excellent physical,chemical,mechanical and anti-corrosion properties,shows high specific strength and biocompatibility.These all excellent characteristics make it get more widely used in the medical field.Biocompatible and bioactive implant surfaces is the key and premise for a good combination of implant and human bone tissue.As a bioinert material,Ti-6Al-4V alloy will directly effect bone healing and bonding after implantation,which is not avial to normal function of human body.Therefore,it is imminent for Ti-6A1-4V alloy to improve its bioactivity and develope a Ti-6Al-4V alloy which has stronger bioactivity.In this paper,Ti-6Al-4V alloy are surface modified by adopting the method of biological functionalization.At first,hot alkali treatment and CDI activation process was carried out respectively.Subsequently,bioactive short-peptide RGDS(Arg-Gly-Asp-Ser)is fixed on surface of Ti-6A1-4V alloy.Ti-6Al-4V alloy is gived bioactivity without affecting its mechanical properties,which achieves bioactive fixation between titanium alloy and human tissue.Fourier Transform Infrared Spectroscopy(FTIR)and X-ray Photoelectron Spectroscopy(XPS)are developed to characterize the surface modified Ti-6A1-4V alloy.Confocal Laser Scanning Microscope(CLSM)is developed to characterize its surface roughness,sessile drop contact angle meter is developed to characterize its water contact angle and hydrophilicity,which are further characterization of the surface modified Ti-6A1-4V alloy.Finally,mouse embryoosteoblastprecursor cells(MC3T3-E1)are cultured in vitro.Through immunofluorescence staining and observation,we study on the growth and biologic behavior of MC3T3-E1 cells on the surface of Ti-6A1-4V alloy modified by short-peptide RGDS.The main studies are as follows:Ti-6Al-4V alloy shows larger surface roughness and good performance of hydrophilicity after hot alkali treatment;The surface roughness and hydrophilicity of Ti-OH and Ti-OH-CDI-RGDS are not significantly changed;Bioactive RGDS peptides is bound on the surface of Ti-6Al-4V alloy successfully through hot alkali treatment and CDI activation;MC3T3-E1 cells on Ti-OH-CDI-RGDS have a better adhesion and proliferation than Ti-OH after 48 h.