Study On Construction Of Residual Polyacrylamide Degradation Engineered Slurry In Coal Slime Water

In the coal preparation plant,polyacrylamide is often used as a flocculant to flocculate coal slime water.Polyacrylamide has a large number of active groups in the polymer side chain,so it has good water solubility and flocculation.However,polyacrylamides remaining in the circulating water of the coal preparation plant will affect the adsorption rate of the flotation chemicals.In addition,if the circulating water of the coal preparation plant is not properly treated and is discharged into the environment,it will pollute the groundwater.At present,many scholars have screened microbial strains from the environment for the degradation of wastewater containing polyacrylamide,but the degradation rate of environmental bacteria is low and the growth cycle of strains is longer,which is not suitable for industrial production.Protoplast fusion technology has the advantage of being able to fuse genetic genes of multiple parental strains across species boundaries.Therefore,the use of protoplast fusion technology to construct engineering bacteria that can effectively degrade residual polyacrylamide in circulating water of coal preparation plants can improve the degradation of degrading bacteria.Characteristics have a very important significance.In this dissertation,Rhodopseudomonas spheroides and Bacillus subtilis were used to study the effects of domesticated Rhodopseudomonas spheroides and Bacillus subtilis on the residual polypropylene in coal slime water after acclimation of domesticated Rhodopseudomonas spheroides and Bacillus subtilis.The properties of amides for compound degradation are optimized to optimize the technological parameters for the degradation of residual polyacrylamide in coal slime by the composite strains;the protoplast fusion technology is used to obtain the fusion of Rhodopseudomonas spheroides and Bacillus subtilis.The fusion strain with high degradation rate of polypropylene dilamide was found in the circulating water of coal,and the optimum technological parameters for protoplast preparation were explored;then the effect of residual polyacrylamide degradation in coal slurry on the fusion and complex strains was compared.The test results showed that after a period of acclimation,Rhodopseudomonas spheroides and Bacillus subtilis could be induced into functional bacteria that degraded polyacrylamide;the degradation of PAM with complex Rhodopseudomonas spheroides and Bacillus subtilis The test was combined at the factor level with a "culturing temperature" of 35°C,"pH" of 7,and "spike added volume" of 2 mL.Under the optimum parameters,the degradation rate of polyacrylamide was 39.24%.The optimal factor level of protoplast preparation process was 20% sucrose and 1.0mg/mL lysozyme.The concentration of EDTA was 0.2% and the action time was 60 minutes.Under these conditions,the protoplast formation rate was 63.7% and the regeneration rate was 8.9%.The optimal factors for preparation of protoplasts of Bacillus subtilis were sucrose concentration 20%,lysozyme concentration 1.5 mg/mL,and action time 60 min.Under the above conditions,the protoplast formation rate was 58.5% and the regeneration rate was 9.45%.The final degradation rate of the fusions to polyacrylamide was 53.14% under the optimum degradation conditions of the composite strains.By comparing the degradation effect of fusion polyacrylamide in coal slime water with fusion strains and complex strains,the results showed that the degradation effect of fusion agent on residue polyacrylamide in coal slime water was significantly enhanced,and the protoplast fusion had both good properties of both parents.