Research On The Separation And Analytical Methods Of Non-steroid Anti-inflammatory Drugs

Non-steroidal anti-inflammatory drugs(NSAIDs)are anti-inflammatory,analgesic and antipyretic drugs that do not contain corticosteroids and are widely used in the treatment of various diseases.Among NSAIDs,aspirin,diclofenac sodium(DS),ibuprofen,and naproxen(NPX)are commonly used.In this paper,DS was used as the target analyte and cationic polymeric nanoparticles adsorbent was successfully synthesized.The interactions between NSAIDs molecules and silver nanoparticles with different properties were discussed in the paper.The methods were established by the resonance light scattering(RLS)for the determination DS and NPX,respectively.This paper is mainly composed of the following four parts:In chapter 1,the properties and methods of separation and enrichment of NSAIDs were introduced.The analytical methods of DS and NPX were summarized.The characteristics and applications of nanomaterials were also reviewed.In chapter 2,cationic polymeric nanoparticles adsorbent(PNPs)were synthesized with methacryloyloxyethyl trimethyl ammonium chloride(DMC)and ethylene glycol dimethacrylate(EGDMA)as functional monomer and cross-linking agent.The PNPs were characterized by infrared spectroscopy,scanning electron microscopy and transmission electron microscopy.The thermal stability and specific surface area of PNPs were determined.The conditions of synthesis,adsorption and desorption were optimized,and the adsorption properties of PNPs for several NSAIDs were studied.The adsorption thermodynamics and kinetics of PNPs for DS were discussed.The experimental results showed that the maximum adsorption capacity of PNPs to DS was 334.3 mg/g after 7 min at p H 5~8.The quantitative desorption was required 3 min by using 0.1 mol/L Na OH/methanol(1:1,V/V)as eluent.The adsorption process was monolayer adsorption and controlled by chemical adsorption.The PNPs could be used repeatedly for 6 cycles without significant decrease in adsorption capacity.The enrichment factor was 200.In chapter 3,silver nanoparticles(Ag NPs)were synthesized by reducing silver nitrate with sodium citrate.The spectral characteristics between Ag NPs and DS were studied.The parameters such as p H,concentration of Ag NPs,standing time,electrolyte concentration,and coexisting substances were systematically investigated and optimized,and possible mechanism for the RLS changes of Ag NPs in the presence of DS was discussed.A new method was established for the determination of trace DS by RLS.The linear range was0.01~2.0 mg/L,and the detection limit was 2.9 ?g/L.This method was applied to the determination of DS in tablets and urine samples and the recovery was 96.5%~103%.In chapter 4,the new silver nanoparticles(QCS Ag NPs)were prepared by using quaternary ammonium salt chitosan(QCS)as stabilizer.The effect of QCS concentration on the properties of QCS Ag NPs was studied in detail.The result showed that NPX could change the RLS intensity of QCS Ag NPs solution.A simple and novel method was developed to determine NPX by RLS based on the interaction between QCS Ag NPs and NPX.The linear range was between 0.10 and 100 ?g/L,and the detection limit was 0.03 ?g/L.This method was successfully applied to the determination of NPX in tablets and urine samples,and the recovery was 95.7~102%.