Study On The Separation Of Cyanide And The Separation Of Active Components In Cold Pressed Flax Cake

Linseed meal is the by-product after linseed is conducted oil expression.It is two thirds of raw materials.There are nearly 300000 tons of outputs in the country every year.Linseed meal contains 30%of proteins,1.5%of lignin,7%of fat,60%of polysaccharide and fiber.However,cyanogentic glycosides in linseed meal can limit its utilization.After oil expression,linseed meal only can be used as fodder in limit.The flaxseed cake removal of cyanogenic glycosides are the main method of water boiling method,solvent method,microwave processing method,extrusion method and high pressure method,using the colorimetric detection method of the method of comparative study,the method of cyanide removal rate are 39%and 78%solvent method,microwave method,extrusion method,boiling method 90%93%,high 98%;cyanogenic glycoside content high pressure cyanide removal after 5mg/Kg,much higher than the vegetable protein beverage of cyanogenic glucoside contents less than 0.05 mg/L.In order to improve the removal efficiency of cyanide,the high pressure and boiling method was used to remove the Glucoside from flax cake.By high pressure water boiling method decyanogen single factor and orthogonal experiment,high pressure water boiling method decyanogen optimum conditions:solid-liquid ratio was 1:30,cyanide removal time is 90min,the pressure is 0.172MPa,under this condition,cyanogenic glycoside content had not detected.In order to make use of the protein in cold pressed flax cake,the process of extracting flax protein by high pressure boiling method was studied.Experiment results showed that the influence factors of flax protein extraction rate were:extraction time,solid-liquid ratio,extraction pressure;the best combination process of flax protein is:solid-liquid ratio was 1:35,extraction time 120min,pressure is 0.207MPa,the extraction rate of flax protein reached 70%.In this study,using HPLC-diode array detection method to detect the content of lignan,we found that the relative standard deviation of the peak area of guaiacol detection reached about 9.7%.In order to improve the precision of the detection of lignan,the influence factors of the accuracy of the detection of lignan were analyzed by item by item exclusion method.The results showed that the main factors affecting the precision of the detection were:the distillation method after alkali hydrolysis,the wall hanging phenomenon when the sample was dissolved by methanol,and the use of needle filter.In view of the above factors,the corresponding improvement measures were put forward,so that the relative standard deviation of the peak area of guaiacol detection was reduced from about 9.7%to about 0.95%.This study uses the alkaline hydrolysis and organic solvent extraction combined with alkali alcohol extraction of lignans,this process can reduce the extraction time was shortened by 1/2,hydrolysis time is shortened by 3/4,lignans extraction rate reached 85%,reached a purity of 18%lignans.