Observation Of Bacteriostatic Effect On Chilled Meat By Antimicrobial Extract From Bovin Leukocytes

Antimicrobial peptides(Antimicrobial peptide,AMP)is widely distributed in bacteria,fungi,viruses,and all kinds of plants and animals of substances.As an important part of the organism natural immune defense system,AMP has a natural anti-microbial activity.The mammalian antimicrobial peptides primarily exist in neutrophils,skin and mucosal epithelial cells.Because of its broad-spectrum anti-microbial activity,minimal side effects and roles in regulation innate immunity,so it has good value and development prospects.To explore the crude extract of chilled meat of bovine leukocyte antimicrobial peptides inhibitory effect,including the preparation of antimicrobial peptides of bovine leukocyte crude extract,crude extracts antibacterial activity of antimicrobial peptides determined the minimum inhibitory concentration(MIC),determination of the total number of colonies change and bacterial phase changes during the determination of chilled fresh pork and he crude extract of Lm of bovine leukocyte antimicrobial peptides inhibitory effect antimicrobial peptides observations of the effect of chilled meat preservation.The results showed that bovine leukocyte antimicrobial peptides against gram-negative bacteria(E.coli)and Gram-positive bacteria(Staphylococcus aureus)have obvious antibacterial activity;crude extracts antimicrobial peptides on Escherichia coli and Staphylococcus aureus MIC are 0.619?g/?L.The growth curves of bacteria in fresh meat and normal culture are not the same.The growth curve of bacteria in fresh meat is a bell-shaped curve,then the total number of bacteria increases again.The bacteria species are diversity in fresh meat,firstly various mesophilic bacteria die off,and then various psychrotrophic bacteria grow and become the dominant flora..The roles of antimicrobial peptides are lower than predicted results.The reason may be that the distribution of bacteria in fresh meat changes with storage time.The surface bacteria mainly contain Pseudomonas bacteria,spores spp and Acinetobacter spp,and so on.With the change of storage time,the Pseudomonas phase become the dominant bacteria and result in cold meat spoilage.After using the crude extract antibacterial peptid,the total number of colonies of bacteria is significantly lower(P <0.05)than the control group,and the inhibition rate is maintained at 60% to 99%,even as high as of 99%.The results showed that crude extracts of bovine leukocyte antibacterial peptide can inhibit the proliferation of bacteria in frozen meat,frozen meat stored period may be extended,with good inhibitory effect.The results showed that the crude extract of Lm of bovine leukocyte antimicrobial peptides inhibitory effect,the MIC is also 0.619?g/?L.In this study,Lm was artificially added to the chiled meat and placed in a low temperature environment preservation,the results showed thatt at 4 ?,the test group which smeared antimicrobial peptides in Meat Lm inhibition of growth was obviously received(P <0.05),it can reduce the pollution from Lm effectively.