| In this study,PPD and PPT in ginseng leaves were separated by AB-8 macroporous adsorption resin firstly;then ginsenoside F2 was subjected to silica gel column;thereafter,ginsenoside F2 and Rd were transformed into ginsenoside C-K by ginsenosidase type I;then Rb1 was used to confirm the most efficient way to be transformed into C-K by ginsenosidase type I.;at last,the hydrolysis of ginseng extractum on PPD was studied.PPD and PPT were eluted from the AB-8 by two different densities of ethanol respectively.Results showed that ginsenoside Re and Rg1 took the major part in the lower density(36%)alcohol eluent,and there were ginsenoside Rd,F2 as well;the higer ethanol(60%)resolved most Rd,F2,a few Rg1,and no Re.50 g ginseng leaves saponin sample was separated by silica gel column and got the purified F2 10g,with a yield of 20%.Followed HPLC result showed the purity of the purified ginsenoside F2 was 93.01%.Ginsenoside Rd and F2 both can be transformed into C-K.Due to ginsenosidase type I,an induced enzyme from sp.48,ginsenosede Rb1 was firstly transformed into Rd,then F2,CK at the end.In case of transforming Rb1 into C-K,the opitimal condition for ginsenosidase type I was that ginsenodidase reacted with 1%substrate Rb1 for 3-4 hours at 60℃.The complex component in Ginseng extractum can hydrolyze PPD.The majority product was Rg3,as well as Rh2. |
