| SsS is 6-ketones steroidal saponins from Smilax scobinicaulis.It have significant biological activities on seed germination,seeding growth,et al.This article establish a quantitative determination method for SsS,confirm the separation an purification method by biologic test.Developing the 20%SsS wettable powder by filtrate and optimize the auxiliaries.Then,we processed 20%SsS wettable powder and evaluated the quality and indoor efficacy.Conclusion are as follows:1.The proposed method is UV-VIS spectrophotometry.The process parameters are as follows:Chromogenic system is anisaldehyde and concentrated sulphuric acid II,the maximum absorption wavelength was 429 nm.Meanwhile,the central composite design-response surface methodology(CCD-RSM)was used to optimize the conditions.The optimal temperature was 82.4 ℃,the best reaction time was 24 min,and 1.2 mL chromogenic agent was fitted.Choosing ethanol reflux extraction method to extract SsS from the root of Smilax scobinicaulis.The extraction duration is 90 min at 70 ℃,solid to liquid ratio of 1:85 repeat thrice.Suspended the ethanol extract in water,extraction with petroleum ether€ethyl acetate and n-butanol in sequence.Purification the n-butanol section with D101 macroporous resin,elution the saponins completely by 95%ethanol,evaporation the ethanol,then,obtain the ethanol extract.2.Study on the formulation of SsS,developing the formula through filtrate and optimize the auxiliaries,the final formula is SsS 20%,nekal BX 12%,sodium tripolyphosphate 5%,sodium lignosulphonate 3%,silica 60%.3.Detection the quality of 20%SsS wettable powder.The results showed that the sample of 20%SsS WP achieve the national or FAO standard:quality suspension rate is 74.6%;the moist time is 29 s;the mean value of pH is 6.782;fineness is 99.85%(through 325 sieves);the foaming is less than 1 mL.Meanwhile,after hot storage and cold storage,the powder is flowable and without caking or agglomeration.4.Evaluating the indoor efficacy of 20%SsS WP by radish biology test.It has a significant influence on thickening radish hypocotyl.The fittest concentrate of 20%SsS WP is 1.0×10-5 moL/L。But,there is no obvious function on the lengths of hypocotyls,and the weight of radish cotyledons. |
