Degradation Effect Of Pseudomonas Fluorescens F1 On Quinclorac And Analysis Of Related Proteins

Quinclorac,a Quinolinic acids phytocide,possesses a long residual period in the field,dangers the productivity of crop in the following season.This paper studied the high-efficiency degradation strain P.fluorescens,which was which was maintained in laboratory.As Pseadomonas fluorescens,P.fluorescens could take Quinclorac as its sole carbon and energy sources.First,selected the solid microbial inoculum carrier of P.fluorescens from turf,bran,activated carbon powder and activated carbon grain.Bran presented the highest bacteria sucking quantity,turf the second;the preserved effect of P.fluorescens in bran and turf first rised then fell,but the bran presented the higher total bacterial count.Synthesizes each kind of situation,the bran could be taken as the most suitable carrier.Second,A pot experiment was to study the morpholine acid the residues dichloroquinoline soil applied degrading bacteria in the quinclorac contaminated soil degradation,soil catalase soil peroxidase and soil urease,soil dehydrogenase activity and tobacco agronomic traits,detection F1 strains quinclorac repair effect.F1 strains in soil containing quinclorac quinclorac degradation rate.With the degradation of the bacteria increase in the amount of increase to deal with the A3(10%)degradation rate;adding different concentrations of quinclorac in soil degradation of the amount of bacteria,the degradation rate with soil dichloroquinoline morpholine acid concentration increased,decreased,which deal with the B1(0.05 mg·kg-1)degradation rate.Applied degrading bacteria can increase the activity of several enzymes,and soil enzyme activity increased with the degradation of the increase of the amount of bacteria.Third,F1 strains detected by SDS-PAGE protein expression differences in the FI strain in the the quinclorac stress,get three possible quinclorac degradation-related bands,one of the phthalate dioxygenase oxygenase molecular size consistent.Enzyme gene extraction,PCR amplification,the amplification results show that the F1 strains phthalate dioxygenase.Last,the two-dimensional electrophoresis to detect in Quinclorac stress,F1 strains of Protein Expression,select the the quinclorac stress expression was significantly higher than the 21 protein spots in the normal state of MS detection,and functional annotation.Learn more about F1 strains in the the quinclorac stress physiological conditions.