Electrochemical Application Based On Novel Pt-based And Pd-based Nanoalloy

The technology of electrochemical immunoassay has become one of the most important methods for life science,clinical diagnosis and pharmaceutical research.Furthermore,Pt-based and Pd-based nanoalloy were wildly used into electrochemical immunosensor due to its excellent conductivity,catalytic performance and good biocompatibility,which could greatly enhance the sensitivity of the electrochemical immunosensor and cut down time for immediately detection.Consequently,novel Pt-based and Pd-based nanoalloy with different morphology and sizes were prepared,which were used to establish sandwich-type electrochemical immunosensors for sensitive detection of tumor biomarkers with broad detection limits and high sensitivity due to its unique electrochemical property.?1?Carcinoembryonic antigen?CEA?as a kind of tumor marker of wide-adaptability was wildly applied into diagnosis and therapeutic effect,progression of disease and estimation of prognosis for colorectal cancer,breast cancer and lung cancer.So,it is very important application for CEA of rapid and sensitive detection,which could be established a novel immunosensing strategy based on Pt@Pd@Ru nanoalloy.In this work,Pt@Pd@Ru was uniformly loaded on the surface of graphene oxide,which could be coated secondary antibody?Ab₂?as nonoprobe.Then,amino functionalization of iron oxide was utilized to conjugate with primary antibody?Ab₁?as immunosensing platform.After immunoreaction,a sand-witch type electrochemical immunosensor was established for CEA sensitive detection with a broad detection range?0.1 pg/mL⁹ ng/mL?and low detection limits?0.05 pg/mL?.This method was simple and clear,which could possess application value.?2?Alpha fetoprotein?AFP?was an important cancer marker,which was used to diagnose liver cancer by assaying.So,it is necessary to develop a method for sensitive,accurate and rapid detection of AFP.In this paper,a kind of novel nano-alloy?Au@Pt?was prepared using P127 as inhibitor.And then Au@Pt was loaded onto the surface of functionalization of graphene sheets?NH₂-GS?to obtain a new nanocomposite?NH₂-GS-Au@Pt?.Finally,the nanocompositewascombinedwithantibodytogetthenanoprobe?NH₂-GS-Au@Pt-Ab₂?.Polydopamine functionalization of reduced graphene oxide?PDA-rGO?as electrochemical immune sensing platform was used to capture antibody.After immunoreaction,nanoprobe was modified onto the electrode to get an electrochemical immunosensor for detection of AFP.The detection ranges of this immunosensor was from 0.1pg/mL to 10 ng/mL and detection limits was 0.05 pg/mL.The proposed immunosensor was designed clever and presented high sensitivity,which was an important gist for AFP instantly detection.?3?Prostatic cancer has been become one of the most extensive cancer and prostate-specific antigen?PSA?was an important index in early cancer diagnose.So,it is necessary to establish a novel electrochemical immunosensor for sensitive and rapid detection of PSA.In this work,novel Pt-Cu nanoalloy was prepared by using polyvinylpyrrolidone?PVP?as growth inhibitor.Next,Pt-Cu nanoalloy was combined with antibody to obtain a novel nanoprobe?Pt-Cu-Ab₂?.Ferroferric oxide loaded onto the reduced graphene?Fe₃O₄-RGO?as electrochemical immune sensing platform was used to capture antibody.After immunoreaction,nanoprobe was modified onto the electrode to get an electrochemical immunosensor for detection of PSA.The detection ranges of this immunosensor was from 0.1 pg/mL to 5 ng/mL and detection limits was 0.05 pg/mL.?4?Aniline could be of oxidative polymerization by HRP in absence of H₂O₂ at a weak acidic condition to obtain polyaniline?PANI?.In this work,Novel Pd-Cu nano-alloy was prepared which presented high enzyme-like activity towards H₂O₂.Based on this,we deceloped a novel electrochemical immunosensor which used to sensitive detetion of CEA.The detection ranges of this immunosensor was from 0.1 pg/mL to 10 ng/mL and detection limits was 0.03pg/mL...