Application Of Single Heating Source Simultaneous Distillation Extraction On Tobacco Volatiles Analysis

Simultaneous distillation extraction instrument is widely used on extracting the volatile components of tobacco.This study observe the performance of a new SDE instrument——the single heating source SDE instrument(SH-SDE),and compare with the double heating source SDE instrument(DH-SDE)’s performance.The volatile components of tobacco was extracted by SH-SDE and detected by GC-MS and UV-Scan.Use the TIC atlas and UV-Scan atlas to identify the attribute of different fields and parts.The results as follows:1 Three different volatile compounds were extracted by SH-SDE.The extraction efficiency of these volatile compounds is above 85%and the RSD is blow 5%.This study shows the SH-SDE can have a high extraction efficiency and good stability.2 The best project of SH-SDE to extract tobacco volatile compounds is:the ratio of sample and distilled water is 1:25(10g tobacco sample and 250ml distilled water),20g NaCl,extract 3h can get the most amount of extracted tobacco volatile compounds.3 Use the DH-SDE and SH-SDE to extract the same tobacco sample and analyzed the volatile compounds by GC/MS under the same parameter condition.The results show 56 compounds can be detected by SH-SDE and 40 compounds can be detected by DH-SDE.4 Use SIMCA-P13 to modeling the UV-Scan atlas and TIC atlas of volatile compounds by SH-SDE.The results show the PLS-DA model can well recognize different fields by TIC atlas and UV-Scan atlas.5 Use the GC/MS Postrun Analysis to analyze the different volatile compounds of tow production places.The results show that 5 different volatile compounds are found of two places,they are beta.-Damascone,(5Z)-6,10-Dimethyl-5,9-undecadien-2-one,.alpha.,2,2,6-tetramethyl-Cyclohexenebutanal,2,6,10,14-tetramethylpentadecane,Phytane.6 The convenience of useful are be compared between tow SDE instruments through experimental process in this study.The results shows the SH-SDE is more convenience,smaller space usage,less reagent,lower cost of production than DH-SDE.This study showsThis study shows SH-SDE have good stability and high extraction efficiency.SH-SDE is more convenience than DH-SDE.UV-Scan and TIC can recognize the different regions of Luzhou tobacco.But the progress to get UV-Scan is more convenience than TIC.