Bioinformatics Study On Genes Related To Neurodegenerative Diseases Post TBI

Background:Traumatic brain injury(TBI)can cause a variety of sequelae,including neurodegenerative diseases,which impose a huge burden on families and society.Immune-mediated neuroinflammation can persist for several years after TBI,which profoundly affects the clinical prognosis of brain injury.It is of great value to understand the pathogenesis of long-term sequelae of brain injury.Objectives :The purpose of this study was to identify the changes of gene expression characteristics after brain trauma,identify signal pathways,and preliminarily explore the neuroinflammation and neurodegenerative changes after brain trauma.Methods : Data were downloaded and included from the Gene Expression Omnibus(GEO)database,and differential expressed genes(DEGs)were screened by R software.On this basis,GO enrichment analysis,KEGG pathway analysis,signal network analysis and transcription factor network analysis were carried out for differentially expressed genes.The TBI model of mice was established.Hippocampal tissue and peripheral blood samples of mice were taken to detect the expression profile of their mRNA by high-throughput sequencing.Bioinformatics analysis was carried out to verify the changes of neuroinflammation and neurodegeneration after TBI.Outcomes:Two data sets were obtained from GEO database.6,513 DEGs were identified(6,464 up-regulated and 49 down-regulated).Among them,Foxo3,Apoe,Mapt and Trem2 genes were significantly up-regulated.KEGG and GO analysis showed that leukocyte transendothelial migration,chemokine signaling pathway and neurotrophic factor signaling pathway were significantly up-regulated in TBI.These conclusions weresupplemented by high-throughput tissue sequencing in the acute phase of TBI.Conclusion:Through bioinformatics analysis,we have proved that TBI is associated with neuroinflammation and neurodegeneration.Targeting genes including FOXO3,APOE,MAPT and TREM2 may be related to the pathological process after TBI.The expression of electron transport chain in mitochondria may be related to neurodegeneration and other sequelae in TBI patients,which may provide potential therapeutic targets for TBI.