Study On Growth Inhibition Effect Of Enhalus Acodoides Extracts On HABs Species

Research on eutrophication prevention methods in the tropical and subtropical coastal waters and research on biological inhibition technologies of harmful red tide algae is becoming a hot issue in marine ecology research.This paper uses the tropical seagrass plant Enhalus acodoides which collected from the Xincun Bay in Hainan Province as the donor material,using the water extracts?ethanol extracts and hexane extracts of the Enhalus acodoides to test the growth of four common species of red tide algae(Scrippsiella trochoidea?Phaeoeystis glolosa?Karenia mikimotoi and Alexandrium tamarense).The results showed that:(1)The water extract of E.acodoides had a significant(p<0.05)inhibitory effect on S.trochoidea?A.tamarense?K.mikimotoi and P.glolosa.The inhibitiory rates of the four kinds of algae were 95.34%?99.18%?94.4% and 72.46% respectively.(2)The ethanol extracts of E.acodoides had significant(p<0.05)inhibitory effects on S.trochoidea?K.mikimotoi and P.glolosa,the maximum inhibitiory rates of the three kinds of red tide algae reached 78.55% ? 95.82%,and 77.72% respectively.(3)Hexane extract of E.acodoides had only significant inhibitory effect(p<0.05)on S.trochoidea,the maximum inhibition rate reached 58.28%;There was no significant inhibition(p>0.05)effect on A.tamarense?K.mikimotoi and P.glolosa.(4)The water extracts of E.acodoides have some influence on the antioxidant(MDA,ROS)and enzyme system(SOD,GSH)of four species of red tide algae,and can significantly(p<0.05)increase the MDA content of four species of red tide algae;which increased the SOD activity of S.trochoidea?P.glolosa,and K.mikimotoi and decreased the SOD activity of A.tamarense;significantly(p<0.05)incresed the ROS levels of S.trochoidea?A.tamarense and K.mikimotoi.