Identification And Analysis Of Key Biomarkers In Cholangiocarcinoma From Bioinformatics Analysis

Purpose: The candidate biomarkers in cholangiocarcinoma(CHOL)were identified by bioinformatics to provide a possible target for early diagnosis,treatment and prognosis of CHOL.Methods: Three gene microarray datasets and three MicroRNA microarray datasets were downloaded from Gene Expression Omnibus(GEO)database.Differentially expressed genes(DEGs)and differentially expressed MicroRNAs(DEMs)were identified.Functional annotation and enrichment analysis of DEGs was performed by using the Database for Annotation,Visualization and Integrated Discovery(DAVIA).The proteinprotein interaction network(PPI)of DEGs was constructed and drawn,and the most significant module in the PPI networks was identified.Target genes of DEMs were identified using three databases.The hub gene and the MicroRNA associated with it were obtained via overlap among DEGs and target genes of DEMs.Subsequently,the survival analyses and expression profiles of hub genes were analyzed and displayed using Gene Expression Profiling Interactive Analysis(GEPIA).The survival analyses and expression profiles of DEMs were analyzed and displayed using the online database OncomiR.Results: A total of 152 DEGs and two DEMs were identified.Functional annotation and enrichment analysis indicated that DEGs were primarily enriched in xenobiotic metabolic processes,lipid transport and epoxygenase P450,chemical carcinogenesis,biosynthesis of amino acids and PPAR signaling.The most significant module in the PPI networks contains 15 genes,which were mainly enriched in PPAR signaling pathways,complement and coagulation cascades.The hub gene and the MicroRNA associated with it may be involved in the development of CHOL after survival analysis and expression profiles.Conclusion: We obtained 152 DEGs and two DEMs after comprehensive analysis for multiple expression profiles which may help in the diagnosis and treatment of CHOL.In addition,our findings suggested that MMP11 contributed to CHOL progression by acting as a target gene of has-miR-125a-5p.