Study On The Phase-locking Relationship Between LFP And Spike At Mouse's Primary Visual Cortex

In mammalian primary visual cortical neurons,the non-classical receptive field nCRF will modulate the classic receptive field CRF,which is the neural basis for many visual perception phenomena.The phase characteristics(especially phase synchronization characteristics)of Local Field Potentials(LFP)is an important measure to express external stimulus.The phase-locking relationship between the distribution of neuronal Spike and LFP rhythms reflects the neural coding information.However,few studies had linked the relationship with the interaction of nCRF and CRF.A singlechannel electrode recording system was used to model the anesthetized mice,this paper explored the influence of nCRF(peripheral)interaction of CRF(center)the phase-locking relationship between LFP and Spike.The evaluation indexes are phase-locking cell number,phase-locking intensity and phase angle change in the collective ranges of CRF and nCRF.This paper studied the phase-locking relationship between Spike and LFP under different stimulation area conditions(different introduction of CRF and nCRF),different orientations of the center and periphery and different cell types.According to the experimental results,we had the following conclusions:(1)The interaction between nCRF and CRF is obviously reflected in the phaselocking relationship between Spike and LFP in low gamma frequency band.The influence of nCRF on CRF is mostly inhibition or even inversion,which is related to the fact that nCRF is mostly inhibition type.Therefore,the nCRF of visual neurons can be analyzed from the perspective of phase-locking.(2)When there is stimulation in the CRF(area integration method),the trend of the number of phase-locking cells and the phase-locking intensity have the opposite trend with the stimulation area.When CRF hasn't stimulated(central covering method),number and strength have the same trend with the stimulation conditions.Both number and strength reflect whether Spike distribution is concentrated in LFP's phase,so whether or not CRF stimulus has an important effect on the degree of phase-locking.(3)The phase-locking angle will change when nCRF' area has stimulus,but it is also related to whether there is a stimulus in the CRF.The change of the phase-locking angle is of great significance for the subsequent study of phase synchronization coding.(4)The phase-locking relationship between Spike and LFP is not only related to the interaction between nCRF and CRF,but also related to cell type.The number of phaselocking cells,phase-locking intensity and phase angle of inhibition type and facilitation type cells are different.These results are great significance for further understanding the transmission rules and integration mechanism of neural information in the cerebral cortex.