Genome-wide Analysis Of TCP Gene Family In Switchgrass(Panicum Virgatum L.)

Studying the salt-tolerance mechanism of plants is a popular way on the biological improvement of saline soil.The plant-specific transcription factor TCPs play multiple roles in plant growth,development and stress responses.However,genome-wide analysis of the TCP gene family and its role in salt stress have not been announced in switchgrass(Panicum virgatum L.).This paper will study from two aspects.On the one hand,42 PvTCP genes(PvTCPs)are identified from the genome of switchgrass,38 members can be anchored to its chromosomes unevenly.It is also predicted that 9 members are microRNA319(miR319)targeting genes.In addition,they can be divided into three clades based on phylogenetic and conserved domains.Members of the same clade have similar genetic structure and motif localization.Although all PvTCPs can be expressed in different tissues we analyzed,their expression profiles are different under normal conditions.Specific expression may suggest different roles in plant growth and development.In addition,about 20 cis-acting elements were detected in the promoter region of PvTCPs,and 40% were associated with stress responses under salt stress.At the same time,the expression profile of PvTCPs under salt stress was also analyzed.It was found that 29 members responded to salt stress.On the other hand,TCP transcription factors in the post-transcriptional level is still regulated by miR319,so,we constructed a miR319 overexpression vector,and successfully obtained a positive plant material for later analysis.In conclusion,in this paper,the PvTCP gene family was analyzed at the genomic level and post-transcriptional level,and its possible functions in salt stress were initially explored,which laid a foundation for further analysis of the functional analysis of PvTCPs in switchgrass.