The Mycorrhizal Structure Types And AMF Diversity Of Common Plants Of Liliaceae In Inner Mongolia Grassland

Liliaceae in the Cronquist plant taxonomy system is a very complex flora,and its taxonomic levels and attribution have always been hot topics of taxonomic controversy;some genera of Liliaceae are classified as family/subfamily taxon in the APG classification system Arbuscular mycorrhizal fungi can develop into different types of mycorrhizal(Arum-type,Paris-type,Intermediate-type)with more than 80%of terrestrial vascular plants including Liliaceae,and show difference at the family level.This article took Liliaceae plants in Inner Mongolia as the object,observed and compared the mycorrhizal structure types of plant genera and species,to explore the mycorrhizal evidence for the classification of Liliaceae plants.At the same time,morphological and molecular biological methods were used to determine the composition and diversity of AMF communities in the roots and rhizosphere soils of Liliaceae,and their relationship with soil factors.The results were as follows1.The 22 plants of 10 genera common in the Liliaceae are all AM symbiotic plants,and their AM mycorrhizal structure has differences at the genera level.12 species in Allium,Convallaria,and Polygonatum have mycorrhizal structure of Arum type,accounting for 54.5%;6 plants from Fritillaria,Hemerocallis,Lilium and Paris are of the Paris type,accounting for 27.2%;The mycorrhizal structure of 4 plants of Maianthemum,Asparagus,Veratrum are type ?,accounting for 18.3%The results of the tentative classification according to the mycorrhizal structure types are a bit more sympathetic to APG IV classification system2.The AM mycorrhizal infection rate of Liliaceae showed the differences among different genera and species.The average infection rate of Allium plants(except for the extremely low infection rate of Mongolian leek<1%)is 47%,followed by Hemerocallis 26%,Asparagus 12%,Convallarial2%,Lilium 11%,Maianthemum 11%,Paris 10%While Fritillaria,Polygonatum and Veratrum are lower,the infection rates of them are 6%,2.7%and 3.5%,respectively.The mycorrhizal infection rate of Allium is significantly different from other genera(p<0.05).This supports the separation of Allium plants from Liliaceae3.From the rhizosphere soil of Liliaceae plants,4 families,8 genera,52 species of AM fungi spores' morphology species were identified:20 species from Acaulosporaceae's 2 genus,3 species from Claroideoglomeraceae's 1 genus,26 species from 4 genus of Glomeraceae,and 3 species from 1 genus of Gigasporaceae.Glomus is the dominant genus,A.bireticulata,C.etunicatum,F.mosseae,G aggregatum are common species.The density of AMF spores in the rhizosphere soil of Liliaceae is 28?198/20g soil,species abundance is 3?13 species/20g soil,Shannon Wiener index:0.96?2.41,Simpson index:0.57-0.89,Pielou evenness index:0.67-0.934.The OTU obtained by high-throughput sequencing of Liliaceae plants'roots can be annotated with 10 families 13 genera 113 species of AMF,Glomus is the dominant genus.The number of annotated species in Allium is the highest,and the Paris genus has the least annotated species The AMF diversity of root system is:Shannon index 0.30?3.23,Simpson index 0.06-0.84,Chao1 index 9.67?206.945.AMF of Plant community soil high-throughput sequencing OTU annotated a total of 89 species in 11 genera of 10 AMF families.Glomus is the dominant genera.The genetic diversity of soil AMF in plant communities is:Shannon index 1.162?4.119,Simpson index 0.036?0.429,Chaol index 27.440?319.269.Plant community soil high-throughput sequencing OTU annotated 35 Phyla,98 classes,253 orders,451 families,891 genera bacteria;diversity manifested as Shannon index 6.134?6.792,Simpson index 0.00335?0.00823,Chaol richness index is 2753?4280?6.The pH range of rhizosphere soil of Liliaceae is:4.67?8.05,the content of ammonium nitrogen is 6.321?59.680 mg·g-1,availabl e potassium is 6.464?30.795 mg·g-1,available phosphorus is 9.070?9.741 mg·g-1.S_UE range is:65.994?1011.150 ?g·d-1·g-1;S_CAT rang e:13.530?57.050 ?mol·d-1·g-1;S_SC range:42.146?219.096 mg·d-1·g-1;S_ACP range:11.732?21.220?mol·d-1·g-1.7.Correlational analysis showed that:S UE is significantly correlated with AMF's 3 Indexes(p<0.05);S_C AT is significantly correlated with spore density(p<0.05);There is no significant correlation between soil factors and AMF diversity in plant roots.The AMF spores'Pielou evenness index is significantly positively correlated with the AMF's Shannon index(p<0.05),and is significantly negatively correlated with the Simpson index(p<0.01).The Shannon index and Chaol index of bacteria in the soil of the plant community are very significantly positively correlated with the AMF Shannon and Chaol index of the corresponding community soil(p<0.01),but the correlation to the AMF Simpson index is significantly negative(p<0.01).The results indicate that the types and structure of AM have a certain reference value for the classification of Liliaceae plants;The AMF community composition and diversity of liliaceae are abundant,Liliaceae AMF community has biogeographical distribution characteristics;AMF diversity is affected by factors such as soil factors,diverity of bacterica etc..The research in this article also provides a basis for the protection and utilization of grassland biological resources.