| The survival pressure of microorganisms comes from the environment or competitors.phenotypic plasticity of microorganisms allows microorganisms to express different phenotypes to cope with environmental pressures.In contrast,there are few studies and reports on phenotypic plasticity under competition.In this research,several methods including evolutionary culture,NGS and bivariate GWAS were used to compare the plasticity of S.aureus single growth and competitive growth with E.coli,which make a preliminary exploration in this regard.In this research,different strains of S.aureus were co-cultivated for 12 days under single-culture and co-culture with the same strain of E.coli to create a competitive environment.The growth abundance was determined by every 24 hours,and the growth curve of each S.aureus strain was plotted under two conditions.Based on the growth data of the S.aureus at different sampling points and the SNPs detected by whole-genome resequencing,several significant SNPs were obtained by bivariate genome-wide association studies.Among the significant SNPs,some SNPs genes are functionally related to the growth of S.aureus and has been reported,such as gdp P,flr,yyc I,com A,gmp and Cisc R,whose expression level reflect the function of plasticity.SNPs genes express hypothetical protein,are also regarded as may have a effect on the competitive growth of S.aureus.In the meantime,the growth abundance of significant SNPs was compared to found that the genotype changes have little effect on the growth of S.aureus under the competitive environment,which demonstrated the influence of growth plasticity.In conclusion,this research proves that S.aureus has the phenotypic plasticity in terms of competitive growth,and the growth plasticity of S.aureus can help it maintain its stability under competition.In addition,this research advances the study of bacterial growth plasticity and demonstrates the potential of bivariate GWAS for bacterial phenotypic plasticity research. |
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