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Proteomics Response And Degradation Performance Enhancement Of Achromobacter Xylosoxidans GYP4 Under Polybrominated Diphenyl Ethers Stress

Posted on:2021-05-10Degree:MasterType:Thesis
Country:ChinaCandidate:J WangFull Text:PDF
GTID:2370330611965623Subject:Environmental engineering
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Polybrominated diphenyl ethers(PBDEs),a widely used brominated organic compounds,is commonly used in construction materials,electronic products,plastic products and other materials.And it's easily released into the environment during the informal treatment of waste materials.Over the years,these compounds have been gradually found in soil,air,dust,water,sediments,animals and plants.Not only that,the presence of PBDEs has also been detected in the human body.These compounds pose great risks to the ecological environment and adversely affect the thyroid,liver,development,and nerves of the organism.Because the biodegradation of PBDEs has the advantages of green and low cost,it has been widely studied.But the biological characteristics of bacteria in the degradation process are less researched.In addition,because the microorganisms cannot fully adapt to the environment,the bacteria will be likely to be in the "Viable but Non-culturable"(VBNC)state,resulting in the degradation of bacterial function.And studies have shown that the Resuscitation promotion factor(Rpf)can promote these bacteria.In view of this,Achromobacter xylosoxidans GYP4 was selected as the experimental strain in this study,and the protein level mechanism of the strain in the process of degrading 2,2?,4,4?-tetrabromodiphenyl ether(BDE-47)was studied.In addition,the effect of Rpf on BDE-47 degradation and the growth of GYP4 was investigated.The following results and conclusions were obtained:(1)Quantitative analysis of i TRAQ proteome showed that 415 differentially expressed proteins were identified during the process of strain GYP4 degrading BDE-47,including 167 up-regulated proteins and 248 down-regulated proteins.Through comprehensive analysis of bioinformatics,it was found that the impact of the degradation process on strain GYP4 was mainly related to multiple metabolic pathways(such as carbohydrate metabolism,lipid metabolism,amino acid metabolism,etc.),and also affected the transport of strain GYP4 and the response to stress etc.In addition,a number of differential protein spots related to VBNC were found,indicating that the activity of the strain GYP4 was significantly inhibited when the bacteria were cultured again after long-term low-temperature storage.(2)Rpf can promote the degradation of BDE-47 and the growth of GYP4: the degradation rate of BDE-47 by the strain GYP4 increased with the increase of Rpf(0?10%(v/v)).When the addition of Rpf reached 10 %(v/v),the degradation efficiency of BDE-47 could increase from 19.85% to 64.91% at 96 h when the initial concentration of BDE-47 was 1 mg/L.Meanwhile,the addition of Rpf can shorten the lag period of strain GYP4,thus the strain GYP4 entered the stable period earlier,and also delayed the arrival of the decay period.At the same time,the number of bacteria also increased in the entire biodegradation process.(3)The cell surface characteristics of the strain GYP4 during the degradation of BDE-47 after adding the Rpf showed that: during the degradation of BDE-47 by the strain GYP4,Rpf accelerated the update rate of peptidoglycan on the surface of strain GYP4,so it affected the permeability and surface hydrophobicity of bacteria.Thereby Rpf promoted the contact between strain GYP4 and BDE-47.
Keywords/Search Tags:2,2?,4,4?- tetrabromodiphenyl ether, Biodegradation, Proteomics, Resuscitation promoting factor
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