IL-17A Promotes The Differentiation Of Bone Marrow Mesenchymal Stem Cells Into Neuronal Cells

Objective This study aimed to investigate whether interleukin 17A(IL-17A)may have the ability to promote neural differentiation of mesenchymal stem cells(MSCs)in vitro and study related molecular mechanisms.Methods First,rat bone marrow mesenchymal stem cells(BMSCs)were cultured in vitro using the whole bone marrow adherent method.Then the neural differentiation induction medium was used to simulate the process of neurogenic differentiation,and IL-17A(Concentration settings are 5 ng / m L,10 ng / m L,20 ng / m L,30 ng / m L,40 ng / m L)at different concentrations was applied to rat BMSCs.RT-q PCT and morphological analysis were used to determine the optimal concentration of IL-17 A to promote neural differentiation of bone marrow mesenchymal stem cells.Subsequently,we continued to induce the neural differentiation of BMSCs with the optimal concentration of IL-17 A on the basis of neurogenic differentiation.RT-q PCR and WB were used to detect the expression of neural-related genes and proteins Tubulin,Hes-1and Thy-1.The Giemsa staining and immunofluorescence staining were used to investigate the morphological changes of the cells and the expression of the neurogenic marker Thy-1.In addition,we still use RT-q PCR and WB to detect the expression of genes and proteins related to neural pathways Jagged-1,Notch-1,Wnt3 a and ?-catenin.Then,the morphological changes of cells and the expression of Thy-1 and CD24neuron-specific markers were still investigated by Giemsa staining and immunofluorescence staining.Images were processed and analyzed using GraphPad Prism 6.01 software,all data are expressed as mean ± SE.The P value we obtained was less than 0.05 and was considered statistically significant.Results RT-q PCT and morphological analysis were used to determine that the optimal concentration of IL-17 A to promote neural differentiation of bone marrow mesenchymal stem cells was 20 ng / m L.Using RT-q PCR,WB,Giemsa staining,and immunofluorescence staining,we determined that IL-17 A at 20 ng / m L has a better effect on promoting the differentiation of rat bone marrow mesenchymal stem cells into neuron-like cells.Mechanistically,we found that during the neural differentiation process,the Wnt/ ?-catenin signaling pathway is activated and the Notch signaling pathway is inhibited.In addition,these two signaling pathways have a certain antagonistic role in regulating IL-17A-induced BMSCs neurogenic differentiation.Conclusion IL-17 A with an optimal concentration of 20 ng / ml has a strong ability to promote rat bone marrow mesenchymal stem cells to differentiate into neuron-like cells.Mechanistically,the Wnt / ?-catenin signaling pathway is activated and the Notch signaling pathway is inhibited related to this differentiation process.