Visualization Of Small Molecules And Heat Based On Organic Probes

Visualization technology is a method that transforms effective information into visualized information(e.g.imaging).At present,the visualization of endogenous substances in cells is mainly achieved through the combination of using fluorescent probes and imaging equipment,i.e.,fluorescence microscope.The labeled endogenous substances are recognized by imaging tecnhiques.However,the development of new specific recognition groups is time-consuming.Therefore,it is useful to extend the application of existing specific recognition groups.As a result,different fluorescent probes with the same recognition group can respond to diverse targets.In addition to the visualization of substances,the visualization of common physical quantity such as sound and temperature,is also important.Therefore,we aim to develop novel temperature visualization system ased on organic probes.The main research contents of this thesis are as follows:1)Design of fluorescent probe for the visualization of sulfite.A novel fluorescent probe NRH-NBD containing nitrobenzofuran group was designed and synthesized for detecting sulfite,a typical endogenous signal substance.The application of NRH-NBD in sulfite sensing was investigated,and the results showed that NRH-NBD is specifically responded.In addition,NRH-NBD has the advantages of good sensitivity,low detection limit(70 nM),and wide linear range(2.0-100 ?m).Also,the actual sample detection of white wine and the imaging of exogenous and endogenous in cells were achieved.This work not only proposes a sulfite probe,but also applies H2S-responsive NBD group into sulfite sensing.Thus,it extend the application of NBD group.2)Constrution of temperature visualization system.In this chapter,a known thermochemiluminescent molecule,acridine-based 1,2-dioxetane,was synthesized.And then,a programmable logic control(PLC)temperature control device was assembled and we studied various characteristics of acridine-based 1,2-dioxetane as a temperature probe.The results showed that acridine-based 1,2-dioxetane has good sensitivity and resolution in comparison with conventional organic fluorescent temperature probes,and its linear response range is 85-130 ?.In addition,there is no need of excitation light source,compared with common fluorescent temperature probes,which reduces light interference.In addition,we can applied this temperature detection system to quick screening of insulation performance of insulation materials.