Construction Of Biomimetic Nanochannel With Active Small Biomolecule Response In Life Based On Chemical Reaction Strategy

In nature,there are various unique functional structures present in life.Scientists continued to study these complex physiological processes,which could provide new ideas for the development and application of biology and materials science.As an important element in the living body,ion channels have an indispensable role in generating signals and maintaining the homeostasis of the cells.thereby they act as an important object for scientific community to study life activities.It will not only help people understand the complex process of nature,but also promote the development of chemistry,biology,materials and other disciplines.Ion channels in the body can selectively transport ions across cell membranes through changes in physiological conditions.At the same time,they also have three basic characteristics such as ion selectivity,ion gating,and ion rectification.These are closely related to most biological functions such as energy conversion.Inspired by this,bionic artificial nanochannels have attracted widespread attention from scientists due to its high in vitro stability and other advantages.Biomimetic nanochannels are usually responsive to the target substance through the weak interaction,but this weak interaction makes the constructed biomimetic nanochannels achieve identification of the target substance through the highly specific chemical reaction.However,the construction biomimetic nanochannels through simple chemical reaction strategies to simulate ion channels in living organisms is still a challenging task.Although there have been several studies on reaction based nanochannels,there are relatively few studies on how to apply these systems to regulate active small biomolecules from nature.At the same time,the physiological role of active small biomolecules(NO,CO,ONOO-)in the living body and the life processes involved in regulating ion channels have been widely reported.The construction of artificial nanochannels for the response of active small biomolecules from nature not only promotes our understanding of the processes of life,but also has important significance in the field of the construction of biomimetic materials platforms.Based on these issues,this paper studies the following two systems:(1)A CO-responsive biomimetic nanochannel was constructed by introducing functional molecules ethylenediamine and 4-nitro-1,8-naphthalenedioside into the nanochannel.The nanochannel realizes the regulation of the surface charge based on the CO-specific redox reaction,thereby realizing the switching state of the channel.This system demonstrates high specificity,stability and recyclability,which provides a certain theoretical basis for the realization of high sensitivity and high specificity detection of CO and nano-sensors.(2)Recent studies have confirmed that ONOO' plays a vital role in living organisms.For example,it can regulate biological ion channels and thereby hyperpolarize smooth muscle.Therefore,we modified rhodamine-110 and hydrazine hydrate in the wall surface of nanochannel to construct a biomimetic nanochannel that responds to ONOO' with high specificity.This system realizes the regulation of the channel switching state through the chemical reaction including the open and close of the spiral ring.It not only helps us understand the process of ONOO-regulating ion channels in life,but also provides a certain research basis for fields such as biomedicine.