Adsorption Ability Of Di-n-butyl Phthalate By Lactobacillus Plantarum P1 Studies

Lactic acid bacteria(LAB)is generally recognized as safe microorganisms with a variety of probiotic functions,and is often used to solve food safety problems related to chemical harmful substances pollution.Phthalic acid esters(PAEs)of environmental estrogens of is a kind of environmental toxic organic compounds.As such compounds are difficult to degrade and migrate,they can easily enter into animal and plant bodies through soil,water and air,and finally accumulate in human body through food chain,causing harm to human health.In recent years,new functions of lactic acid bacteria have been found,such as adsorbing heavy metal ions,mycotoxins and so on.Therefore,we can use lactobacillus to solve the harm of PAEs compounds to human body.In this study,eight strains of lactic acid bacteria were isolated for their ability to detoxify Di-n-butyl Phthalate(DBP),and one of the strains,Lactobacillus plantarum P1,was selected for more detailed analyses of its phthalate adsorption capacity in vitro.This study also evaluated the in vivo protective effects of strain P1 against DBP toxicity in rats.The strain P1 was selected through in-vitro experiments,which has a high adsorption ratio for DBP,and the adsorption ratio reached 60.8%±7.6%,and it had adsorption stability.Through in-vivo experiments in rats,it was found that P1 can significantly reduce the concentration of monobutyl phthalate(MBP)in urine and increase the concentration of DBP in feces.Furthermore,estrogenic effects of the different treatments were assessed through counting of sperm and observation of testis,and strain P1 could protect the sexual organs of male rats.Our results suggested that P1 can adsorb DBP,and be against phthalate toxicity in vivo and could be considered as a new dietary therapeutic strategy against environmental phtalate toxicity.This study also investigated the exopolysaccharides(EPS)production of P1.Through genetic engineering,lactobacillus plantarum P1 was modified to increase its exopolysaccharides production by 11.3 mg/L and improved its DBP adsorption capacity,this provides more applications possibilities for lactic acid bacterias.