Detection And Preliminary Analysis Of Antibody Level Against Avian Influenza Virus And Newcastle Disease Virus Of Wildfowl From Wild Zoo

At present,researchers at home and abroad have done little research on wild animal diseases.Wild birds are the natural hosts of Newcastle disease virus(NDV)and Avian Influenza virus(AIV).It is also a potential carrier of NDV and AIV and a source of infection.In order to prevent and control the epidemic diseases of safari birds and understand the current immunization status of safari birds,this study was carried out.Hemagglutination and hemagglutination inhibition tests were used to detect the antibody levels of birds in Hefei Wild Zoo and Zhengzhou Zoo before and after vaccine immunization.A total of 207 wild birds from Hefei Wild Zoo and Zhengzhou Zoo were selected.There are 35 peacocks,20 white pheasants,8 pheasants,5 white-bellied pheasants,9red-bellied pheasants,2 guinea fowls,3 turkeys,6 ring-necked pheasants,9 white-crowned pheasants,red Two Belly-horned Pheasants,17 White Cranes,13 White-naped Cranes,10 Red-Crowned Cranes,14 Grey Crowned Cranes,17 White Storks,23 Flamingos,2 Red Ducks,2 Hongyans,4 Swan,There are 4 mandarin ducks and 2 barred geese.Vaccination of two diseases for all wild birds during fall prevention in 2019.The hemagglutination(HA)and hemagglutination inhibition(HI)test methods were used to detect the H5,H7 and H9 subtypes of avian influenza and Newcastle disease antibodies before immunization,30 d and 60 d after immunization,respectively.The titers of wild bird H5,H7,H9 subtype avian influenza and Newcastle disease before immunization were 6.4log2,5.9log2,6.6log2 and 5.6log2,respectively.The titers of H5,H7,H9 subtype avian influenza and Newcastle disease antibodies before immunization of Phasianidae wild birds were 6.2log2,6.0log2,5.9log2 and 4.8log2,respectively,and H5,H7,H9 subtype avian influenza and Newcastle birds Antibody titers were 7.5log2,5.8log2,7.8log2 and 7.1log2,respectively,and the titers of H5,H7,H9 subtype avian influenza and Newcastle disease pre-immunization were 5.6log2,6.0log2,6.7log2 and 5.9 log2,the antibody titers of H5,H7,H9 subtypes of avian influenza and Newcastle disease before duck immunization were 6.2log2,5.7log2,6.6log2 and 4.9log2.The antibody titers of 10 wild birds H5 subtype avian influenza were lower than 4 log2,4wild bird H7 subtype avian influenza antibody titers were lower than 4 log2,5 wild bird H9 subtype avian influenza antibody titers were lower than 4 log2,33 wild bird Newcastle disease antibody titers were lower than 4 log2.The antibody titers of avian influenza H5,H7,H9 subtypes and Newcastle disease were10.1log2,10.0log2,10.0log2,and 9.6log2,respectively,30 days after immunization,and H5,H7,H9 subspecies 30 days after immunization The antibody titers of avian influenza and Newcastle disease were 10.0log2,10.0log2,9.9log2 and 9.6log2,respectively,and the antibody titers of H5,H7,H9 subtypes of avian influenza and Newcastle disease were10.3log2 30 days after vaccination of Helicopteridae,10.2log2,10.3log2 and 9.9log2.The antibody titers of H5,H7,H9 subtype avian influenza and Newcastle disease were10.0log2,9.8log2,10.1log2 and 9.1log2 respectively 30 days after vaccinations of storks.The antibody titers of H5,H7,H9 subtypes of avian influenza and Newcastle disease were9.7log2,9.7log2,9.9log2 and 9.4log2 30 days after immunization of duck poultry.The antibody titers of H5,H7,H9 avian influenza and Newcastle disease in wild birds60 days after immunization were 8.9log2,9.0log2,9.1log2 and 7.7log2,respectively,and60 days after immunization of wild birds of Phasianidae H5,H7,H9 subtype The antibody titers of avian influenza and Newcastle disease were 8.7log2,9.0log,8.9log2 and7.4log2,respectively,and the antibody titers of H5,H7,H9 subtype avian influenza and Newcastle disease were 9.3log2 60 days after vaccination of Helicidae wild birds,9.3log,9.6log2 and 8.7log2,antibody titers of H5,H7,H9 subtype avian influenza and Newcastle disease 60 days after immunization of stork-shaped wild birds are respectively 8.7log2,8.5log2,9.0log2 and 6.8log2,Anatidae The antibody titers of H5,H7,H9 subtype avian influenza and Newcastle disease were 9.1 log2,8.9 log2,9.3 log2,and 7.8 log2 60 days after wild bird immunization.The results showed that the levels of antibodies to avian influenza and Newcastle disease among different species of wild birds before immunization were quite different,and the levels of antibodies among individuals of the same species were quite different.The antibody titers were high at 30 d and 60 d after immunization,and no stress response occurred.It is safe and effective for wild birds to be immunized with poultry vaccines.The same vaccine and immunization program have different immunization effects on different wild birds,and it is necessary to develop a practical and safe and effective immunization program for the immune antibodies of wild birds.