Study On The Characteristics Of Rhizospheric Microbial Communities In The Heterogeneous Habitats Of Coastal Wetlands In The Yellow River Delta

Soil microorganisms are key components of terrestrial ecosystems and play a vital role in soil formation,decomposition of fallen matter,material circulation and energy flow,ecological restoration,etc.Microorganisms,with their unique living conditions,ecological roles and functional divisions,are susceptible to changes in the soil environment,so microbial diversity is often considered one of the important indicators for evaluating soil changes.The spatial distribution patterns of soil microorganisms that enhance ecosystem stability and their response to soil physicochemical factors have been the focus of research in recent years.The coastal wetlands are a unique and complex ecosystem arising from the intersection of land and sea,with important ecological functions and resource values,and exploring the structure and diversity of soil microbial communities in coastal wetlands can deepen our knowledge and understanding of the importance of soil microbes in typical ecosystems.The Yellow River Delta is China's warm-temperate,most extensive and complete estuarine wetland ecosystem,with fragile habitats.strong heterogeneity,and obvious water-salt gradients,which has attracted the attention of many scholarsIn this study,the structural characteristics of plant rhizosphere microbial communities in different habitat reed communities(from the intertidal zone to the Yellow River coast)and their influence mechanisms were investigated using the Yellow River Delta coastal wetland ecosystem as the study area,heterogeneous habitat reed communities as the study object,and soil microbes as the main line of study.The high-throughput sequencing of reed rhizosphere soil microbial communities in heterogeneous habitats in the coastal wetlands of the Yellow River Delta was used to investigate the structural characteristics of the reed rhizosphere bacteria,fungi,and mycorrhizal root fungus communities,and to further analyze the response patterns of reed rhizosphere soil microbial communities to soil environmental changes by combining soil pH,electric conductivity,soil organic matter content,soil total nitrogen content,soil total phosphorus content,soil nitrate and ammonium nitrogen content.soil available potassium content.Based on the biodiversity index of soil microorganisms,a correlation analysis and redundancy analysis are combined to investigate the impact of soil physiochemical properties on the diversity of soil microbial communitiesIn general,soil nitrate nitrogen and organic matter are the main factors affecting the structure of the rhizosphere microbial community in the coastal wetlands of the Yellow River Delta.The main findings and conclusions are as follows.A total of 57 phyla,140 classes,394 orders,680 families,1309 genera and 2805 species of bacteria were detected,including Proteobacteria that was dominant,and the diversity of the bacterial community was sensitive to soil pH.And the abundance of Deltaproteobacteria,Chloroflexia and Kazania were significantly different among the samples.A total of 10 phyla,32 classes,74 orders,172 families.302 genera and 417 species of fungi were detected,among which the dominant fungi was Ascomycota.And the main fungus with significant difference in abundance in soil was Glomeromycota.A total of 5 orders,8 families,10 genera and 54 species of mycorrhizal fungi were found,and the dominant AMF belongs to the genus GlomusThe Yellow River Delta wetlands are a unique ecosystem affected by a combination of fresh and salt water,providing an ideal research case for comprehending the environmental impact on the structure and function of soil microbes in coastal ecosystems.The results of this study contribute to a better understanding of the rhizosphereed soil microbial community of plants in the coastal wetlands and the soil physicochemical factors that influence the AMF community.