Promoting Effect Of Ginger From Rhizosphere Probiotics

China is the country with the largest ginger planting area and the largest total production in the world,and Shandong Province is the largest planting area in the north.However,with the promotion of planting,many problems have gradually become apparent,mainly due to the problems of environmental pollution and soil degradation caused by excessive fertilization or other improper control during the control process.Therefore,a new and low-pollution scientific method needs to be found to replace the traditional.The microbial inoculant can increase the output of agricultural products while preventing environmental pollution,improving the environment and soil quality,and achieving sustainable development of agricultural production.The development of a special microbial inoculum for ginger is conducive to the green and efficient production of ginger.This study focused on the identification and functional screening of four different ginger rhizosphere-promoting strains.The effect of different inoculants on the growth-promoting effects of ginger was studied,and the effects of the inoculants on the community structure of ginger rhizosphere microorganisms were discussed..After morphological observation,physiological and biochemical analysis,and 16S rDNA sequence analysis,the four strains were identified as Bacillus licheniformis LCDD6,B.thuringiensis TA-201,B.thuringiensis TA-202 and B.mycoides TA-203.The protein-decomposing,iron-producing carriers and other ability of the four strains were tested.LCDD6,TA-201,TA-202,and TA-203 had the ability to produce IAA,protein-decomposing,iron-producing carriers,and phosphorus.The specific primers of antibiotics were amplified by PCR to detect whether the four strains contained iturin A,bacillomycin D,fengycin,surfactin,pyoluteorin gene,LCDD6 detected four antibiotic synthesis genes,TA-201,TA-202 and TA-203 detected two antibiotic synthesis genes.To verify the growth-promoting effect of four strains of ginger,we carry out a pot test,and the application of a single bacteria agent TA-201,TA-202,TA-203,LCDD6 and a compound bacteria(LCDD6,TA-201,TA-202 and TA-203 bacterial volume ratio of 1:1:1:1)agent can increase the plant height of ginger by 14.32%-49.60%and the stem diameter of ginger by 11.63%-34.05%.The fresh weight of ginger above ground increased by82.22%-150.92%and the above-ground dry weight was increased by 83.58%-188.06%.Comprehensive agronomic traits and biomass,LCDD6 in a single bacterial agent has the best growth promotion effect on ginger and the compound bacteria on ginger has a better effect on promoting growth than single bacteria.Studies on the effect of inoculants on the bacterial community structure of ginger soil in pots found that except for the Treat2 treatment group,the Shannon,Simpson,and Chao1 indexes were higher than the control group in the five treatment groups with the inoculum application,indicating that the inoculum application,to a certain extent,it can increase the richness and diversity of the bacterial community structure.In order to verify the effect of the compound fungus on the ginger yield,a field test was carried out.In the field test,it was found that the treatment group with bacterial fertilizer JF had the highest ginger yield.Compared with the control group CK,the ginger yield per mu increased by 517.87kg,and the increase accounted for CK.16.92%of the treatment group;the YF of the treatment group applying the bacterial fertilizer and the bacterial agent increased266.67kg per mu of ginger compared with the control group CK applying the compound fertilizer,and the increase accounted for 8.69%of the CK;the treatment group JY applied the bacterial agent Compared with the control group of compound fertilizer,the output of ginger per mu decreased by 33.33kg,accounting for 1.11%of CK.The high-throughput sequencing results showed that the dominant bacterial communities of the ginger rhizosphere soil in the field were Proteobacteria,Actinomycetes,Campylobacter,Acidobacteria,Bacillus,Bacteroides,and Pachyphyta.The dominant phylums of the ginger rhizosphere soil fungal community in the field were Ascomycota,Basidiomycota,and Flagellates.PCoA and hierarchical cluster analysis showed that the bacterial and fungal community structures were separated in different treatment groups,which indicated that the application of different fertilizers could significantly change the ginger rhizosphere microbial community structure.Genome sequencing analysis of B.licheniformis LCDD6,which has the best effect of ginger growth,and found that the growth-promoting genes related to the production of auxin,phosphorus dissolution,and iron-producing vectors and lichenysin,bacillibactin and other antibiotic genes.These will provide very important value for us to introduce LCDD6 to the microbial inoculants application later.When bean sprout juice is used as the basic medium,the carbon source,nitrogen source,inorganic salt,and pH value are optimized progressively(the optimized nutrients are added to the next nutrient optimized medium).When glucose is the carbon source,the concentration of LCDD6 bacteria can reach 1.035×10~9 cfu/mL,when soybean meal is the nitrogen source,the concentration of LCDD6 bacteria can reach 2.665×10~9 cfu/mL,and the concentration of LCDD6 bacteria can reach 3.61×10~9cfu/mL,the concentration of LCDD6 bacteria can reach4.00×10~9 cfu/mL when the pH of the medium is 8.0.This study provides a theoretical basis for the use of microbial inoculants to increase ginger yield and lays the foundation for the development of strain resources for ginger-specific microbial preparations.