The Isolation And Identification Of Feline Parvovirus JL-3 Strain And Establishment Of Animal Infection

Cats are one of the most favorite pets and are distributed throughout the world.As the spiritual companion of human beings,the health of cats have gradually aroused people's attention.Cat plague,also called feline panleukopenia,is one of the most important infectious diseases that is a common infectious disease caused by Feline panleukopenia virus?FPV?.At present,vaccination and supportive therapy are the mainly prevention and control methods.However,experimental animal models are required for both the evaluation of the immune effects of vaccines and the evaluation of the therapeutic or therapeutic efficacy of health drugs.Therefore,the purpose of this study was to isolate the virus from clinical materials,establish a cat plague infection model,and provide help for clinical vaccine development and therapeutic drug evaluation.It is of great clinical significance and practical value to carry out this research?In this study,the anus swabs of clinically suspected feline panleukopenia were collected and tested by PCR.The positive samples were repeatedly freeze-thawed for 3 times after being treated with normal saline and inoculated with F81 cells.The virus was identified by cytopathology observation,electron microscopy negative staining observation,TCID50 detection,FPV VP2 gene sequencing and evolutionary tree analysis.In order to further study the pathogenicity of FPV isolates,the best infection route and the best challenge time were tested.The experimental model of FPV infection in cats was preliminarily established by observing clinical symptoms,body condition score,changes of temperature and weight,jejunal histological changes and detoxes in feces.The results showed that an FPV strain,named FPV JL-3 strain,belonged to group G 1 strain and was closely related to the current epidemic strains in China and surrounding countries.The experimental cats were inoculated with 10^4.51 TCID₅₀/mL FPV JL-3 strain.The best way of challenge was oral administration,and the best dose was 1.5 mL.On day 3 after inoculating,the virus could be detected in the anal swab by PCR,but not on day 35.Conclusion:10^4.51 TCID₅₀/mL FPV JL-3 strain was used to inoculate the Chinese garden cat orally with a dose of 1.5 mL and no detoxification at 35 days,which was used as the model of cat parvovirus infection.