Cell Research Based On Micro-nano Interface Regulation Of Material Surface

The spread of cancer throughout the body is driven by circulating tumor cells?CTCs,Circulating tumor cells?.These cells detach from the primary tumor and move from the bloodstream to a new site for subsequent tumor growth.They carry information about the primary tumor and have the potential to be valuable biomarkers for disease diagnosis and progression,and for the molecular characterization of certain biological properties of the tumour.However,the limited sensitivity and specificity of current methods for measuring and studying these cells in patient blood samples prevents the realization of their full clinical potential.Most cell-material interactions are mediated by biologically active interfaces,so it is important to understand the materials that make up the interface and how they function.Recent studies have developed several nanomaterial-based biointerfaces that attempt to increase the efficiency of CTC capture.However,the sensitivity and selectivity of CTCs-specific recognition in these nanomaterials is limited.The specific research work of this thesis is as follows:1.Using the"bottom-up"model method,a TiO₂ nanowire substrate with a micro-structure and a nanostructure-like"honeycomb"microstructure interface was prepared,on the surface cancer cells culture and adhesion morphology were investigated.2.TiO₂ nanowires were prepared on the surface of micro-grooves by one-step hydrothermal method,cancer cells and fibroblasts was cultured on the surface and research their adhesion morphology.The preparation process of the micro-nano substrate is highly controllable,and as the hydrothermal time increases,the density of the TiO₂ nanowire becomes larger and the longer.3.An organic electrochemical transistor sensor?OECT?based on graphene oxide?GO?biointerface was prepared for cancer cell detection.By doping GO into the OECT active layer,the rough texture of the GO enhances the topographical interaction between the CTC and the biological interface.Moreover,the contact area of the CTC adhered to the OECT organic active layer can be improved,which helps to improve the CTC capture efficiency.In addition,specific antibody modification is performed on the surface of the active layer of OECT to specifically capture and detect circulating tumor cells.