Study On The EST-SSR Development And Genetic Diversity Of Artemisia Intramongolica H.C.Fu Based On Transcriptome

Artemisia intramongolica H.C.Fu is found in Hunshandake sandy land and is endemic to China.In this paper,the transcriptome sequencing of A.intramongolica was carried out,SSR primers were developed,the distribution characteristics of SSR were analyzed,and the genetic diversity and genetic structure of A.intramongolica were studied by using the selected SSR primers,The main results are as follows:1.SSR was searched based on all 84183 Unigenes,of which 9784 sequences contain a total of 1148 SSR,a total length of 55,956,737 bp.The distribution frequency of SSR is 13.64%,the frequency of occurrence is11.62%,and the distribution density is 4.87 kb.The largest number of SSR repeat types were mononucleotide,trinucleotide and dinucleotide,and the SSR density distribution was also mononucleotide>trinucleotide>dinucleotide.The 8~10,5 and 6 repetitions of mononucleotide,trinucleotide and dinucleotide were the most repeated,and the most repeated primitive sequences were A/T,AC/GT and ATC/ATG.10~19bp has the largest number of SSR loci in the repeat length type.2.After 94 individuals capillary electrophoresis of A.intramongolica by15 pairs of primers,81 alleles were identified,and 5.4 alleles were identified on average per loci.A total of 266 genotypes and 132 specific genotypes were identified,with an average specific genotype ratio of 49.63% per bit.3.The desired hybridization He is 0.63,Shannona Diversity index I is1.82,and PIC of A.intramongolica species level is 0.58,which is greater than0.5,reflecting the high polymorphic information content.The variation of the desired hybridization is 0.60~0.65,the I value is between 1.67~1.89,and the variation of the polymorphic information content PIC of the population level is 0.54~0.60,the overall performance has a high level of diversity,and there islittle difference between the various groups,all three indexs had the highest population diversity in Inner Mongolia Zhenglanqi Sanggendalai(SGDL)and Xilin Hot Grassland Ecosystem Location Research Station(DWZ)in Inner Mongolia,Chinese Academy of Sciences.4.The genetic differentiation Hpop/Hsp in the population of A.intramongolica was 86.02%,the genetic differentiation among populations(Hsp-Hpop)/Hsp was 13.98%,it shows that 86.02% of the genetic variation exists in the population,and 13.98% of the genetic variation exists among the populations.The genetic distance between the various groups is between0.0368~0.0913,indicating that the genetic distance is small.The genetic distance between SGDL and XSQ was closest to 0.0368.By using MEGA6,the cluster diagram of UPGMA type was plotted according to Nei's genetic distance,which showed that the population of SGDL and XSQ were the first to gather in one branch,the population of Xilin Hot BLKMC formed a branch alone,the population of DWZ and SY formed another branch.After Mantel test,there was no significant correlation between genetic distance and geographical distance.5.The population structure of A.intramongolica was analyzed by Structure 2.3.4 software based on Bayesian algorithm,and the change graph of DeltaK with K was obtained by Structure Harvester software,showing that when K is 3,the DeltaK value is the largest,that is,5 populations of A.intramongolica is from 3 gene banks.Genetic structure analysis shows that the DWZ and SY populations belong to a gene bank,XSQ population belongs to the second gene bank,SGDL and BLKMC populations belong to the third gene bank.